Fluorouracil selectively enriches stem-like cells in the lung adenocarcinoma cell line SPC
| Autor: | Xiao-lei Li, Xin Li, Yan-lei Xiong, Mumu Shi, Enhua Wang, Li Zhang, Xinshan Jia |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Pathology
medicine.medical_specialty Antimetabolites Antineoplastic Lung Neoplasms Cell Blotting Western Apoptosis Biology Adenocarcinoma Immunofluorescence Stem cell marker Colony-Forming Units Assay Mice Side population Cancer stem cell Cell Movement medicine Biomarkers Tumor Tumor Cells Cultured Animals Humans Fluorescent Antibody Technique Indirect Side-Population Cells Cell Proliferation Mice Inbred BALB C medicine.diagnostic_test Cell Cycle General Medicine Cell cycle Molecular biology medicine.anatomical_structure Neoplastic Stem Cells Female Fluorouracil Stem cell Adult stem cell |
| Zdroj: | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 34(3) |
| ISSN: | 1423-0380 |
| Popis: | Most adult stem cells are in the G0 or quiescent phase of the cell cycle and account for only a small percentage of the cells in the tissue. Thus, isolation of stem cells from tissues for further study represents a major challenge. This study sought to enrich cancer stem cells and explore cancer stem-like cell clones using 5-fluorouracil (5-FU) in the lung adenocarcinoma cell line, SPC. Proliferation inhibition was analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, according to which half maximal inhibitory concentration values were calculated. Expression levels of stem cell markers after treatment with 5-FU were examined using immunofluorescence and Western blotting. Additionally, side population (SP) cells were sorted using FACS. Properties of SP cells were evaluated by using Transwell, colony-forming assays, and tumor formation experiments. 5-FU greatly inhibits proliferation, especially of cells in S phase. SP cells possess greater invasive potential, higher clone-forming potential, and greater tumor-forming ability than non-SP cells. Treatment with 5-FU enriches the SP cells with stem cell properties in human lung adenocarcinoma cell lines. |
| Databáze: | OpenAIRE |
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