Selective Expression of the Insulin I Gene in Rat Insulinoma-Derived Cell Lines
| Autor: | Stephen J. Giddings, Lynn R. Carnaghi, Frederick T. Fiedorek |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
Transcription Genetic medicine.medical_treatment Molecular Sequence Data Gene Expression Cell Line Endocrinology Internal medicine Gene expression medicine Animals Humans Insulin RNA Processing Post-Transcriptional Molecular Biology Gene Base Sequence biology Gene Amplification Rat Insulinoma Nucleic Acid Precursors DNA General Medicine Molecular biology IRS2 Rats Pancreatic Neoplasms Kinetics Insulin receptor medicine.anatomical_structure Cell culture biology.protein Insulinoma Pancreas |
| Zdroj: | Molecular Endocrinology. 4:990-999 |
| ISSN: | 1944-9917 0888-8809 |
| Popis: | Four rat insulinoma-derived cell lines were found to express only the rat insulin I gene, although an apparently normal insulin II gene was present in each cell line. This finding was reflected by the absence of insulin II and the presence of insulin I gene transcripts in the products of run-on transcription assays, the absence of insulin II and the presence of insulin I unprocessed (pre-) mRNA, and the absence of mature insulin II and the presence of insulin I mRNA. Analysis of insulin II genes in cell lines by gene amplification indicated that the lack of insulin II gene transcription is not explained by the absence of or gross alterations in the insulin II genes per se. These studies indicate that the two nonallelic insulin genes do not function equivalently in pancreas-derived cells. |
| Databáze: | OpenAIRE |
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