In vitro effect of different cytokinin types (BAP, TDZ) on two different Ocimum basilicum cultivars explants
| Autor: | Erika Kurucz, Miklós Fári, E. Enkhbileg |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
food.ingredient lcsh:Plant culture 01 natural sciences 03 medical and health sciences chemistry.chemical_compound food In vitro 010608 biotechnology lcsh:SB1-1110 BAP (6-Benzylaminopurine) Cultivar 030304 developmental biology 0303 health sciences biology fungi Basilicum food and beverages Sweet Basil lcsh:QK900-989 Ocimum biology.organism_classification food.food Horticulture sweet basil chemistry Ocimum basilicum L Callus cytokinin effect Shoot Cytokinin lcsh:Plant ecology TDZ (Thidiazuron) Explant culture |
| Zdroj: | International Journal of Horticultural Science, Vol 25, Iss 3-4 (2019) |
| ISSN: | 2676-931X 1585-0404 |
| DOI: | 10.31421/ijhs/25/3-4/3930 |
| Popis: | Ocimum basilicum L. (sweet basil) is an economically and ethnobotanically important aromatic, medicinal, ornamental and culinary herb, with a very wide gene pool, that is sensitive to cold and prone to several plant pathogens that can demolish harvest and lessen yield. In this research, the effects of BAP (6-Benzylaminopurine) and TDZ (Thidiazuron) on different genotypes for in vitro cloning were determined, in order to provide a detailed protocol guide concerning Ocimum basilicum L. propagation. The results from the O. basilicum seed propagations revealed that the best condition for the secondary shoot growth is with 5.0 mg/l TDZ or 1.5 mg/l BAP on all types of explants except the root, the secondary root growth can be obtained on all types explant with any BAP concentration and all cytokinins can induce callus on all types of explants. On the whole, it shows that multiple secondary shoot induction and regeneration in Ocimum basilicum L. is regulated by appropriate cytokinin concentration. |
| Databáze: | OpenAIRE |
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