Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells
| Autor: | Kevin A. D'Amour, Kelly G. Ross, Laura Martinson, Evert Kroon, Mike Richardson, Mark A. Moorman, Melissa K. Carpenter, Traci M Ostertag, Emmanuel E. Baetge, Man Yin Chan, Anne G. Bang, Kuniko Kadoya, Olivia Kelly |
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| Rok vydání: | 2011 |
| Předmět: |
Transplantation
Heterologous Biomedical Engineering Bioengineering Embryoid body Cell Separation Mice SCID Biology Stem cell marker Applied Microbiology and Biotechnology Antibodies Mice Cancer stem cell Antigens CD medicine Animals Humans Pancreas Cells Cultured Embryonic Stem Cells Endoderm Flow Cytometry Embryonic stem cell Molecular biology Cell biology medicine.anatomical_structure Microscopy Fluorescence Amniotic epithelial cells Molecular Medicine Stem cell Biomarkers Biotechnology Adult stem cell |
| Zdroj: | Nature biotechnology. 29(8) |
| ISSN: | 1546-1696 |
| Popis: | Using a flow cytometry-based screen of commercial antibodies, we have identified cell-surface markers for the separation of pancreatic cell types derived from human embryonic stem (hES) cells. We show enrichment of pancreatic endoderm cells using CD142 and of endocrine cells using CD200 and CD318. After transplantation into mice, enriched pancreatic endoderm cells give rise to all the pancreatic lineages, including functional insulin-producing cells, demonstrating that they are pancreatic progenitors. In contrast, implanted, enriched polyhormonal endocrine cells principally give rise to glucagon cells. These antibodies will aid investigations that use pancreatic cells generated from pluripotent stem cells to study diabetes and pancreas biology. |
| Databáze: | OpenAIRE |
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