Participation of leukotriene D4 and tumor necrosis factor on lipopolysaccharide-induced airway hyperresponsiveness in guinea pigs
| Autor: | Takashi Uno, Hiroichi Nagai, Hiroyuki Tanaka, Noriko Nakai |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Lipopolysaccharides
Male Serotonin Leukotriene D4 Bronchoconstriction Guinea Pigs Pharmaceutical Science Pharmacology Pentoxifylline Guinea pig chemistry.chemical_compound medicine Escherichia coli Animals Leukotriene Inhalation medicine.diagnostic_test business.industry Tumor Necrosis Factor-alpha General Medicine respiratory system Acetylcholine Recombinant Proteins respiratory tract diseases Bronchoalveolar lavage chemistry Immunology lipids (amino acids peptides and proteins) Tumor necrosis factor alpha Bronchial Hyperreactivity business Hydroxy Acids Bronchoalveolar Lavage Fluid Histamine medicine.drug Interleukin-1 |
| Zdroj: | Biologicalpharmaceutical bulletin. 20(4) |
| ISSN: | 0918-6158 |
| Popis: | In guinea pigs, a marked increase in airway responsiveness to acetylcholine (Ach) was observed at 2 h after lipopolysaccharide (LPS) inhalation. To examine the mediators responsible for the airway hyperresponsiveness, the changes of peptide-leukotrienes (LTs), tumor necrosis factor (TNF), interleukin-1 (IL-1), histamine and 5-hydroxytryptamine (5-HT) levels in bronchoalveolar lavage fluid (BALF) were measured. Airway responsiveness to Ach reached a peak 2 h after LPS inhalation. The influx of neutrophil into BALF increased gradually and reached a peak 24 h after LPS inhalation. After the inhalation of LPS, LTD4 and TNF contents in BALF increased within the first 2 h after LPS inhalation. However, other mediators were not detected or increased 6 h after LPS inhalation. Aeroinhalation of LTD4 and murine recombinant TNF-alpha caused airway hyperresponsiveness in guinea pigs. In addition, a LTD4 antagonist, BAYx7195, and an inhibitor of TNF, pentoxifylline, inhibited the LPS-induced airway hyperresponsiveness. These results suggest that LTs and/or TNF play an important role in the onset of airway hyperresponsiveness in guinea pigs. |
| Databáze: | OpenAIRE |
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