Transcriptional profile of cytokines, regulatory mediators and tlr in mesenchymal stromal cells after inflammatory signaling and cell-passaging

Autor: Robim Marcelino Rodrigues, Makram Merimi, Vera Rogiers, Philippe Lewalle, Laurence Lagneaux, Dhouha Daassi, Rahma Melki, Karolien Buyl, Hassan Fahmi, Mehdi Najar, Joery De Kock, Tamara Vanhaecke
Přispěvatelé: Department of Bio-engineering Sciences, Pharmaceutical and Pharmacological Sciences, Experimental in vitro toxicology and dermato-cosmetology, Vriendenkring VUB
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Regulatory mediators
TLR 1
Transcription
Genetic
Mesenchymal Stem Cells/metabolism
Mesenchymal stromal cells
Informatique appliquée logiciel
Cell-passage
Toll-Like Receptors/genetics
Physico-chimie générale
0302 clinical medicine
Inflammation/genetics
Biology (General)
Spectroscopy
education.field_of_study
Cytokines/genetics
Brief Report
Toll-Like Receptors
General Medicine
Down-Regulation/genetics
Computer Science Applications
Cell biology
Up-Regulation
Transcriptional profile
Chemistry
030220 oncology & carcinogenesis
Cytokines
Tumor necrosis factor alpha
medicine.symptom
mesenchymal stromal cells
Signal Transduction
QH301-705.5
Population
Subcutaneous Fat
Down-Regulation
Inflammation
Biology
Chimie inorganique
Gene Expression Regulation/genetics
Catalysis
CCL5
Inorganic Chemistry
Up-Regulation/genetics
03 medical and health sciences
Cell Proliferation/genetics
Downregulation and upregulation
cell-passage
TLR
medicine
Humans
Spectroscopie [état condense]
Physical and Theoretical Chemistry
transcriptional profile
education
Molecular Biology
QD1-999
regulatory mediators
Cell Proliferation
Transcription
Genetic/genetics
Organic Chemistry
Mesenchymal stem cell
Biologie moléculaire
Mesenchymal Stem Cells
Chimie théorique
cytokines
Chimie organique
030104 developmental biology
Spectroscopie [électromagnétisme
optique
acoustique]
Gene Expression Regulation
Cell culture
Signal Transduction/genetics
Catalyses hétérogène et homogène
Subcutaneous Fat/metabolism
Zdroj: International journal of molecular sciences, 22 (14
International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 22, Iss 7309, p 7309 (2021)
Popis: Adult human subcutaneous adipose tissue (AT) harbors a rich population of mesenchymal stromal cells (MSCs) that are of interest for tissue repair. For this purpose, it is of utmost importance to determine the response of AT-MSCs to proliferative and inflammatory signals within the damaged tissue. We have characterized the transcriptional profile of cytokines, regulatory mediators and Toll-like receptors (TLR) relevant to the response of MSCs. AT-MSCs constitutively present a distinct profile for each gene and differentially responded to inflammation and cell-passaging. Inflammation leads to an upregulation of IL-6, IL-8, IL-1β, TNFα and CCL5 cytokine expression. Inflammation and cell-passaging increased the expression of HGF, IDO1, PTGS1, PTGS2 and TGFβ. The expression of the TLR pattern was differentially modulated with TLR 1, 2, 3, 4, 9 and 10 being increased, whereas TLR 5 and 6 downregulated. Functional enrichment analysis demonstrated a complex interplay between cytokines, TLR and regulatory mediators central for tissue repair. This profiling highlights that following a combination of inflammatory and proliferative signals, the sensitivity and responsive capacity of AT-MSCs may be significantly modified. Understanding these transcriptional changes may help the development of novel therapeutic approaches.
SCOPUS: ar.j
info:eu-repo/semantics/published
Databáze: OpenAIRE
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