Sensitivity of bacterial biofilms and planktonic cells to a new antimicrobial agent, Oxsil® 320N
| Autor: | Marie-Paule Gelle, Dominique Laurent-Maquin, N. Surdeau, S. Bouthors |
|---|---|
| Přispěvatelé: | SODIFRA (SODIFRA), SODIFRA, Interfaces biomatériaux/tissus hôtes, Université de Reims Champagne-Ardenne (URCA)-IFR53-Institut National de la Santé et de la Recherche Médicale (INSERM), Laurent-Maquin, Dominique |
| Rok vydání: | 2006 |
| Předmět: |
Microbiology (medical)
Biocide Silver Exopolymer Disinfectant Microbial Sensitivity Tests MESH: Biofilms Biology Neutralization MESH: Dose-Response Relationship Drug Microbiology 03 medical and health sciences chemistry.chemical_compound MESH: Silver Peracetic acid MESH: Plankton Hydrogen peroxide Acetic Acid 030304 developmental biology [SDV.IB] Life Sciences [q-bio]/Bioengineering MESH: Drug Combinations MESH: Microbial Sensitivity Tests 0303 health sciences Bacteria Dose-Response Relationship Drug 030306 microbiology MESH: Disinfectants Biofilm Hydrogen Peroxide General Medicine Plankton Antimicrobial Drug Combinations MESH: Bacteria Infectious Diseases chemistry Biofilms MESH: Acetic Acid MESH: Hydrogen Peroxide MESH: Drug C [SDV.IB]Life Sciences [q-bio]/Bioengineering Disinfectants |
| Zdroj: | J Hosp Infect J Hosp Infect, 2006, 62, pp.487-93. ⟨10.1016/j.jhin.2005.09.003⟩ |
| ISSN: | 0195-6701 |
| Popis: | Summary The effective concentrations of disinfectants were determined for planktonic bacteria using the norms EN 1040 and NF T 72–150. This concentration corresponds to biocide efficacy after 5min of contact, followed by neutralization. However, micro-organisms often colonize a substratum and form microcolonies or biofilms where they are enclosed in exopolymer matrices. Biofilms are commonly resistant to a broad range of antimicrobial agents, and resistance mechanisms involve exopolymer matrices, changes in gene expression and metabolic alterations. Due to these different resistance mechanisms, it is difficult to select and titrate antimicrobial agents to be effective against biofilms. In this context, SODIFRA developed a new disinfectant, Oxsil ® 320N (French patent 94 15 193). Oxsil 320N is an association of three active principles: hydrogen peroxide, acetic acid/peracetic acid and silver. This biocide was tested on planktonic bacteria and on 24-h biofilms formed on AISI 304 stainless steel surfaces. The effective concentration of Oxsil 320N was also determined on biofilms using SODIFRA recommendations (without neutralization of the biocide). Data showed that the antimicrobial efficacy measured on planktonic bacteria is not a reliable indicator of performance when biofilm is present. When biofilms were exposed to Oxsil 320N, the concentration needed to achieve a 10 5 -fold decrease in concentration was 10 times higher than that for bacterial suspensions (0.313% Oxsil 320N). An effective concentration of Oxsil 320N of 3.13% was required. |
| Databáze: | OpenAIRE |
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