Characterization of microRNA expression in B cells derived from Japanese black cattle naturally infected with bovine leukemia virus by deep sequencing

Autor: Toshihiro Ichijo, Sonoko Miyauchi, Chihiro Ochiai, Syuji Yoneyama, Kazuya Nagai, Yuta Naruke, Yusuke Chiba, Sota Kobayashi, Yuta Kudo, To-ichi Hirata, Leng Dongze, Shinji Yamada, Keisuke Tomita, Kenji Murakami, Hirokazu Hikono
Rok vydání: 2021
Předmět:
B Cells
Lymphoma
animal diseases
viruses
Artificial Gene Amplification and Extension
Biochemistry
Polymerase Chain Reaction
law.invention
Hematologic Cancers and Related Disorders
White Blood Cells
Proviruses
Animal Cells
immune system diseases
law
Medicine and Health Sciences
Leukemia Virus
Bovine
B-cell lymphoma
Polymerase chain reaction
Mammals
B-Lymphocytes
Multidisciplinary
Bovine leukemia virus
biology
Messenger RNA
Eukaryota
virus diseases
Ruminants
Hematology
Viral Load
Nucleic acids
medicine.anatomical_structure
Oncology
Vertebrates
Medicine
Cellular Types
Research Article
Immune Cells
Science
Immunology
Research and Analysis Methods
Deep sequencing
Bovines
microRNA
Genetics
medicine
Animals
Non-coding RNA
Antibody-Producing Cells
Molecular Biology Techniques
Molecular Biology
B cell
Natural antisense transcripts
Blood Cells
Biology and life sciences
Organisms
Cancers and Neoplasms
Cell Biology
Enzootic Bovine Leukosis
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
medicine.disease
Virology
Gene regulation
MicroRNAs
Amniotes
RNA
Cattle
Gene expression
Zoology
Zdroj: PLoS ONE, Vol 16, Iss 9, p e0256588 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL), a malignant B cell lymphoma. However, the mechanisms of BLV-associated lymphomagenesis remain poorly understood. Here, after deep sequencing, we performed comparative analyses of B cell microRNAs (miRNAs) in cattle infected with BLV and those without BLV. In BLV-infected cattle, BLV-derived miRNAs (blv-miRNAs) accounted for 38% of all miRNAs in B cells. Four of these blv-miRNAs (blv-miR-B1-5p, blv-miR-B2-5p, blv-miR-B4-3p, and blv-miR-B5-5p) had highly significant positive correlations with BLV proviral load (PVL). The read counts of 90 host-derived miRNAs (bta-miRNAs) were significantly down-regulated in BLV-infected cattle compared to those in uninfected cattle. Only bta-miR-375 had a positive correlation with PVL in BLV-infected cattle and was highly expressed in the B cell lymphoma tissue of EBL cattle. There were a few bta-miRNAs that correlated with BLV tax/rex gene expression; however, BLV AS1 expression had a significant negative correlation with many of the down-regulated bta-miRNAs that are important for tumor development and/or tumor suppression. These results suggest that BLV promotes lymphomagenesis via AS1 and blv-miRNAs, rather than tax/rex, by down-regulating the expression of bta-miRNAs that have a tumor-suppressing function, and this downregulation is linked to increased PVL.
Databáze: OpenAIRE
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