A reciprocal translocation, induced by a canonical integration of a single T-DNA, interrupts the HMG-I/Y Arabidopsis thaliana gene
| Autor: | Annie Depeiges, Georges Picard, Fabienne Degroote, Jacqueline Lafleuriel |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
DNA
Bacterial Genetics Regulation of gene expression Base Sequence Genotype biology Physiology Molecular Sequence Data Breakpoint Arabidopsis Chromosome Chromosomal translocation Sequence Analysis DNA Plant Science Gene rearrangement biology.organism_classification Chromosomes Plant Translocation Genetic Phenotype Kanamycin Arabidopsis thaliana Coding region HMGA1a Protein Gene |
| Zdroj: | Plant Physiology and Biochemistry. 42:171-179 |
| ISSN: | 0981-9428 |
| DOI: | 10.1016/j.plaphy.2004.01.003 |
| Popis: | Major chromosomal rearrangements occur during Arabidopsis thaliana T-DNA transformation. They generally result from interactions between multiple T-DNA copies during the integration process or from aborted integration events. We report here a reciprocal translocation associated with the integration of a single T-DNA which otherwise shows all the characteristic features of a canonical integration event. The exchanged fragments roughly correspond to half of the left arm of chromosome 1 and to two thirds of the right arm of chromosome 2. The chromosome 1 breakpoint maps close to position 23.6 cM and interrupts the coding sequence of the HMG-I/Y gene, which is present at a single copy in the Arabidopsis genome and encodes a non-histone chromosomal protein putatively involved in regulation of gene expression. The chromosome 2 breakpoint maps close to position 33.6 cM, and is located 419 bp upstream of a gene encoding a putative homeodomain transcription factor. Homozygotes for the translocation display a severe phenotype with major developmental abnormalities and total sterility, while heterozygotes are fertile, most of them showing a wild-type phenotype. Among the six possible unbalanced genotypic classes, four are entirely lethal while only a few individuals from the two others survive. Analysis of relations between phenotypes and genotypes strongly suggests that the major phenotypic alterations observed do not result from inactivation of the HMG-I/Y gene. |
| Databáze: | OpenAIRE |
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