Three-dimensional imaging of intracochlear tissue by scanning laser optical tomography (SLOT)
| Autor: | Tinne, N., Nolte, L., Antonopoulos, Georgios C., Schulze, J., Andrade, J., Heisterkamp, A., Meyer, H., Warnecke, A., Majdani, Omid, Ripken, T., Choi, Bernard, Kollian, Nikiforos, Zeng, Haishan, Kang, Hyun Wook, Wong, Brian J.F., Ilgner, Justus F., Tearney, Guillermo J., Gregory, Kenton W., Marcu, Laura, Skala, Melissa C., Campagnola, Paul J., Mandelis, Andreas |
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| Rok vydání: | 2016 |
| Předmět: |
inner ear
Materials science cochlea Dewey Decimal Classification::600 | Technik::620 | Ingenieurwissenschaften und Maschinenbau tomography 3-D microscopy 01 natural sciences Antibodies Fluorescence law.invention 010309 optics 03 medical and health sciences antibody staining Optical clearing 0302 clinical medicine Optics Optical microscope law 0103 physical sciences Microscopy medicine Scanning Scanning lasers Optical tomography Konferenzschrift Cochlea Visualization Tissue Tomographic reconstruction ?CT medicine.diagnostic_test business.industry Computerized tomography Laser scanning laser optical tomography Autofluorescence 3D microscopy sense organs Tomography ddc:620 business 030217 neurology & neurosurgery SLOT |
| Zdroj: | Proceedings of SPIE 9689 (2016) |
| ISSN: | 0277-786X |
| DOI: | 10.1117/12.2211066 |
| Popis: | The presented study focuses on the application of scanning laser optical tomography (SLOT) for non-destructive visualization of anatomical structures inside the human cochlea ex vivo. SLOT is a laser-based highly efficient microscopy technique, which allows for tomographic imaging of the internal structure of transparent large-scale specimens (up to 1 cm3). Thus, in the field of otology this technique is best convenient for an ex vivo study of the inner ear anatomy. For this purpose, the preparation before imaging comprises mechanically assisted decalcification, dehydration as well as optical clearing of the cochlea samples. Here, we demonstrate results of SLOT visualizing hard and soft tissue structures of the human cochlea with an optical resolution in the micrometer range using absorption and autofluorescence as contrast mechanisms. Furthermore, we compare our results with the method of X-ray micro tomography (micro-CT, μCT) as clinical gold standard which is based only on absorption. In general, SLOT can provide the advantage of covering all contrast mechanisms known from other light microscopy techniques, such as fluorescence or scattering. For this reason, a protocol for antibody staining has been developed, which additionally enables selective mapping of cellular structures within the cochlea. Thus, we present results of SLOT imaging rodent cochleae showing specific anatomical structures such as hair cells and neurofilament via fluorescence. In conclusion, the presented study has shown that SLOT is an ideally suited tool in the field of otology for in toto visualization of the inner ear microstructure. © 2016 SPIE. |
| Databáze: | OpenAIRE |
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