Biological scaffold-mediated delivery of myostatin inhibitor promotes a regenerative immune response in an animal model of Duchenne muscular dystrophy
| Autor: | Liam Chung, Kaitlyn Sadtler, Shoumyo Majumdar, Kenneth Estrellas, Jyothi Mula, Matthew T. Wolf, Lindsay A. Cheu, Kathryn R. Wagner, Jennifer H. Elisseeff |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Duchenne muscular dystrophy Myostatin Muscle Development Biochemistry T-Lymphocytes Regulatory Myoblasts Mice 0302 clinical medicine Drug Delivery Systems Absorbable Implants Myocyte Muscular dystrophy Hyaluronic Acid biology Tissue Scaffolds Chemistry Antibodies Monoclonal Forkhead Transcription Factors Hydrogels Molecular Bases of Disease musculoskeletal system Cell biology Extracellular Matrix medicine.anatomical_structure Mannose Receptor Macrophage polarization Receptors Cell Surface 03 medical and health sciences medicine Animals Humans Regeneration Lectins C-Type Muscle Skeletal Molecular Biology Regeneration (biology) Macrophages Skeletal muscle Cell Biology Muscular Dystrophy Animal medicine.disease Immunity Innate Transplantation 030104 developmental biology Mannose-Binding Lectins Gene Expression Regulation biology.protein Mice Inbred mdx 030217 neurology & neurosurgery |
| Zdroj: | The Journal of biological chemistry. 293(40) |
| ISSN: | 1083-351X |
| Popis: | Recent studies have reported that the immune system significantly mediates skeletal muscle repair and regeneration. Additionally, biological scaffolds have been shown to play a role in polarizing the immune microenvironment toward pro-myogenic outcomes. Moreover, myostatin inhibitors are known to promote muscle regeneration and ameliorate fibrosis in animal models of Duchenne muscular dystrophy (DMD), a human disease characterized by chronic muscle degeneration. Biological scaffolds and myostatin inhibition can potentially influence immune-mediated regeneration in the dystrophic environment, but have not been evaluated together. Toward this end, here we created an injectable biological scaffold composed of hyaluronic acid and processed skeletal muscle extracellular matrix. This material formed a cytocompatible hydrogel at physiological temperatures in vitro. When injected subfascially above the tibialis anterior muscles of both WT and dystrophic mdx-5(Cv) mice, a murine model of DMD, the hydrogel spreads across the entire muscle before completely degrading at 3 weeks in vivo. We found that the hydrogel is associated with CD206(+) pro-regenerative macrophage polarization and elevated anti-inflammatory cytokine expression in both WT and dystrophic mice. Co-injection of both hydrogel and myostatin inhibitor significantly increased FoxP3(+) regulatory T cell modulation and Foxp3 gene expression in the scaffold immune microenvironment. Finally, delivery of myostatin inhibitor with the hydrogel increased its bioactivity in vivo, and transplantation of immortalized human myoblasts with the hydrogel promoted their survival in vivo. This study identifies a key role for biological scaffolds and myostatin inhibitors in modulating a pro-regenerative immune microenvironment in dystrophic muscle. |
| Databáze: | OpenAIRE |
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