A Book-Type Dried Plasma Spot Card for Automated Flow-Through Elution Coupled with Online SPE-LC-MS/MS Bioanalysis of Opioids and Stimulants in blood
| Autor: | Imelda Ryona, Jack Henion |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Quality Control
Bioanalysis Hematocrit Pharmacology 030226 pharmacology & pharmacy 01 natural sciences Analytical Chemistry Automation 03 medical and health sciences 0302 clinical medicine Tandem Mass Spectrometry medicine Humans Whole blood Chromatography medicine.diagnostic_test Elution Chemistry 010401 analytical chemistry Codeine 0104 chemical sciences Dried blood spot Analgesics Opioid Hydrocodone Dried Blood Spot Testing Sample collection Chromatography Liquid medicine.drug |
| Zdroj: | Analytical Chemistry. 88:11229-11237 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | Despite many benefits including simple point-of-care sample collection, reduced costs, and simplified shipping and storage, dried blood spot (DBS) techniques have faced adoption resistance due to factors such as the hematocrit (Hct) effects and the established preference for bioanalysis of plasma rather than whole blood. One way to potentially circumvent these challenges is to adopt the concept of dried plasma spot (DPS) techniques. One approach to accomplishing this is through an on-card red blood cell (RBC) filtration to generate plasma from whole blood without the need for centrifugation. In this report, a book-type DPS card has been developed and validated by employing fully automated flow-through elution coupled with online SPE-LC-ESI-MS/MS for the quantitative determination of four representative opioids (morphine, codeine, oxycodone, hydrocodone) and five stimulants (amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine (MDMA), phentermine, and mephedrone) in one method using their corresponding deuterium-labeled analogues as internal standards. Method validation results showed good linearity (R |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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