Extracellular entrapment and degradation of single-walled carbon nanotubes
| Autor: | Bengt Fadeel, Gregg P. Kotchey, Kunal Bhattacharya, Consol Farrera, Alexander Star, Kjell Hultenby, Fernando Torres Andón, Beatrice Lazzaretto |
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| Rok vydání: | 2014 |
| Předmět: |
Materials science
biology Nanotubes Carbon Neutrophils Carbon nanotube Neutrophil extracellular traps Biodegradation Extracellular Traps Immunity Innate Neutrophil Activation law.invention Catalysis Biochemistry law Myeloperoxidase Extracellular biology.protein Biophysics Humans Degradation (geology) General Materials Science Cells Cultured Ex vivo |
| Zdroj: | Nanoscale. 6:6974 |
| ISSN: | 2040-3372 2040-3364 |
| Popis: | Neutrophils extrude neutrophil extracellular traps (NETs) consisting of a network of chromatin decorated with antimicrobial proteins to enable non-phagocytic killing of microorganisms. Here, utilizing a model of ex vivo activated human neutrophils, we present evidence of entrapment and degradation of carboxylated single-walled carbon nanotubes (SWCNTs) in NETs. The degradation of SWCNTs was catalyzed by myeloperoxidase (MPO) present in purified NETs and the reaction was facilitated by the addition of H2O2 and NaBr. These results show that SWCNTs can undergo acellular, MPO-mediated biodegradation and imply that the immune system may deploy similar strategies to rid the body of offending microorganisms and engineered nanomaterials. |
| Databáze: | OpenAIRE |
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