Monascus-fermented dioscorea enhances oxidative stress resistance via DAF-16/FOXO in Caenorhabditis elegans
| Autor: | Yeu-Ching Shi, Chan-Wei Yu, Vivian Hsiu-Chuan Liao, Tzu-Ming Pan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Antioxidant
DPPH Applied Microbiology medicine.medical_treatment lcsh:Medicine medicine.disease_cause Microbiology Antioxidants chemistry.chemical_compound Model Organisms Insulin Signaling Cascade Molecular Cell Biology medicine Animals Caenorhabditis elegans Proteins lcsh:Science Biology Caenorhabditis elegans Cellular Stress Responses Nutrition Multidisciplinary biology Traditional medicine Dioscorea business.industry lcsh:R food and beverages Forkhead Transcription Factors Animal Models biology.organism_classification Monascus Signaling Cascades Biotechnology carbohydrates (lipids) Oxidative Stress chemistry Caenorhabditis Elegans Fermentation Daf 16 foxo Medicine lcsh:Q business Oxidative stress Transcription Factors Research Article Signal Transduction |
| Zdroj: | PLoS ONE, Vol 7, Iss 6, p e39515 (2012) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | BACKGROUND: Monascus-fermented products are mentioned in an ancient Chinese pharmacopoeia of medicinal food and herbs. Monascus-fermented products offer valuable therapeutic benefits and have been extensively used in East Asia for several centuries. Several biological activities of Monascus-fermented products were recently described, and the extract of Monascus-fermented products showed strong antioxidant activity of scavenging DPPH radicals. To evaluate whether Monascus-fermented dioscorea products have potential as nutritional supplements, Monascus-fermented dioscorea's modulation of oxidative-stress resistance and associated regulatory mechanisms in Caenorhabditis elegans were investigated. PRINCIPAL FINDINGS: We examined oxidative stress resistance of the ethanol extract of red mold dioscorea (RMDE) in C. elegans, and found that RMDE-treated wild-type C. elegans showed an increased survival during juglone-induced oxidative stress compared to untreated controls, whereas the antioxidant phenotype was absent from a daf-16 mutant. In addition, the RMDE reduced the level of intracellular reactive oxygen species in C. elegans. Finally, the RMDE affected the subcellular distribution of the FOXO transcription factor, DAF-16, in C. elegans and induced the expression of the sod-3 antioxidative gene. CONCLUSIONS: These findings suggest that the RMDE acts as an antioxidative stress agent and thus may have potential as a nutritional supplement. Further studies in C. elegans suggest that the antioxidant effect of RMDE is mediated via regulation of the DAF-16/FOXO-dependent pathway. |
| Databáze: | OpenAIRE |
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