Accumulation of High Levels of Monocytic Myeloid-Derived Suppressor Cells Enhances Graft Survival in Almost-Tolerant Kidney Transplant Recipients
| Autor: | Lan-jun Cai, Xiao-Xiao Du, Min Yang, Sheng Chang, Zhonghua Klaus Chen, Yuliang Guo, Bi-Feng Liu, Y.P. Zhao |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Regulatory T cell 030232 urology & nephrology Renal function Peripheral blood mononuclear cell Organ transplantation Immune tolerance 03 medical and health sciences 0302 clinical medicine Immune system medicine Animals Humans Whole blood Transplantation business.industry Myeloid-Derived Suppressor Cells Graft Survival Kidney Transplantation Transplant Recipients surgical procedures operative 030104 developmental biology medicine.anatomical_structure Immunology Myeloid-derived Suppressor Cell Surgery Female Transplantation Tolerance business |
| Zdroj: | Transplantation proceedings. 50(10) |
| ISSN: | 1873-2623 |
| Popis: | Background Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of immature cells that suppress immune responses during organ transplantation and participate in mediating long-term graft survival and immune tolerance in animal transplant models. However, their role in regulating transplant tolerance in human subjects is not well understood. In the present study, we investigated the role of MDSCs in mediating long-term graft survival in almost-tolerant kidney transplant recipients (ATKTRs) and the mechanism(s) responsible for increasing MDSC numbers in these recipients. Methods Peripheral blood mononuclear cells (PBMCs) from whole blood samples were collected from 30 ATKTRs (graft survival, > 10 years after kidney transplant [KTx]) treated with low doses of immunosuppressive drugs and with stable kidney function, 10 short-term graft survival kidney transplant recipients (STKTRs; graft survival, ∼1–3 years post-KTx) with stable kidney function, and 10 healthy donors (HDs). MDSC and regulatory T cell (Tregs) levels were analyzed using multicolor flow cytometry in PBMCs. Results ATKTRs had significantly higher levels of monocytic MDSCs (P Conclusions Accumulation of high levels of MDSCs was observed in ATKTRs. Changes in MDSC levels may play important roles in mediating transplant tolerance and regulating Tregs. Therefore, we propose that MDSCs may be potentially used for recognizing tolerant transplant recipients and guiding dosage reduction for immunosuppressive drugs for KTx. |
| Databáze: | OpenAIRE |
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