Carnitine Acetyltransferase in AgRP Neurons Is Required for the Homeostatic Adaptation to Restricted Feeding in Male Mice
| Autor: | Zane B. Andrews, Sarah Kathleen Haas Lockie, Randall L. Mynatt, Mathieu Méquinion, Alexander Reichenbach, Romana Stark, Moyra B Lemus, Jacqueline Bayliss |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Male mice Biology 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Endocrinology Feeding behavior Corticosterone Carnitine Acetyltransferase Internal medicine Physical Conditioning Animal Brown adipose tissue medicine Animals Homeostasis Agouti-Related Protein Research Articles Caloric Restriction Mice Knockout Neurons Meal Carnitine O-Acetyltransferase digestive oral and skin physiology Plasma levels Feeding Behavior Adaptation Physiological Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure chemistry 030217 neurology & neurosurgery |
| Zdroj: | Endocrinology. 159(6) |
| ISSN: | 1945-7170 |
| Popis: | Behavioral adaptation to periods of varying food availability is crucial for survival, and agouti-related protein (AgRP) neurons have been associated with entrainment to temporal restricted feeding. We have shown that carnitine acetyltransferase (Crat) in AgRP neurons enables metabolic flexibility and appropriate nutrient partitioning. In this study, by restricting food availability to 3 h/d during the light phase, we examined whether Crat is a component of a food-entrainable oscillator (FEO) that helps link behavior to food availability. AgRP Crat knockout (KO) mice consumed less food and regained less body weight but maintained blood glucose levels during the 25-day restricted feeding protocol. Importantly, we observed no difference in meal latency, food anticipatory activity (FAA), or brown adipose tissue temperature during the first 13 days of restricted feeding. However, as the restricted feeding paradigm progressed, we noticed an increased FAA in AgRP Crat KO mice. The delayed increase in FAA, which developed during the last 12 days of restricted feeding, corresponded with elevated plasma levels of corticosterone and nonesterified fatty acids, indicating it resulted from greater energy debt incurred by KO mice over the course of the experiment. These experiments highlight the importance of Crat in AgRP neurons in regulating feeding behavior and body weight gain during restricted feeding but not in synchronizing behavior to food availability. Thus, Crat within AgRP neurons forms a component of the homeostatic response to restricted feeding but is not likely to be a molecular component of FEO. |
| Databáze: | OpenAIRE |
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