A diverse collection of B cells responded to HIV infection in infant BG505
| Autor: | Theodore A Gobillot, Vrasha Chohan, Ruth Nduati, Laura E Doepker, Julie Overbaugh, Hannah L. Itell, Brianna Hennessy, Cassandra A. Simonich, Evan M. Cale, Nicole A. Doria-Rose, Meghan Garrett, Mackenzie M. Shipley |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Medicine (General) Immunogen HIV Infections V3 loop HIV Antibodies General Biochemistry Genetics and Molecular Biology Virus Epitopes R5-920 Antigen Report Humans Amino Acid Sequence Neutralizing antibody BG505 Antibody-dependent cell-mediated cytotoxicity B-Lymphocytes biology V3 polyclonal Antibody-Dependent Cell Cytotoxicity HIV neutralizing antibody Virology infant Antibodies Neutralizing Clone Cells Polyclonal B cell response Child Preschool Immunoglobulin G biology.protein HIV-1 Antibody ADCC |
| Zdroj: | Cell Reports Medicine Cell Reports Medicine, Vol 2, Iss 6, Pp 100314-(2021) |
| ISSN: | 2666-3791 |
| Popis: | Summary Increasing evidence suggests infants develop unique neutralizing antibody (nAb) responses to HIV compared to adults. Here, we dissected the nAb response of an infant whose virus is in clinical trials as a vaccine immunogen, with a goal of characterizing the broad responses in the infant to this antigen. We isolated 73 nAbs from infant BG505 and identified a large number of clonal families. Twenty-six antibodies neutralized tier 2 viruses—in some cases, viruses from the same clade as BG505, and in others, a different clade, although none showed notable breadth. Several nAbs demonstrated antibody-dependent cellular cytotoxicity activity and targeted the V3 loop. These findings suggest an impressive polyclonal response to HIV infection in infant BG505, adding to the growing evidence that the nAb response to HIV in infants is polyclonal—a desirable vaccine response to a rapidly evolving virus like HIV. Graphical abstract Highlights Infant BG505 developed a broad and polyclonal antibody response to HIV-1 We isolate 73 neutralizing antibodies spanning 19 clonal lineages from BG505 Many antibodies mediate cell killing but show limited neutralization breadth V3 is a common epitope target of the BG505 antibodies Although BG505 Envelope trimer is reputable in the HIV field, this infant’s antibody response was unknown. Simonich et al. isolated 73 neutralizing antibodies encompassing 19 clonal lineages from BG505 at 27 months of age. BG505 developed a vast polyclonal antibody response to HIV, an advantageous response to elicit by vaccination. |
| Databáze: | OpenAIRE |
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