Inhibition of human sperm-zona-free hamster oocyte binding and penetration by protein C inhibitor
| Autor: | Francisco España, J. Gilabert, A Romeu, Jaime Sánchez-Cuenca, P J Fernández, Charles H. Muller, Amparo Estellés, M Royo |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
Urology Protein C inhibitor Hamster Peptide In Vitro Techniques Endocrinology Cricetinae medicine Animals Humans Amino Acid Sequence Peptide sequence Zona Pellucida Protein C Inhibitor Sperm-Ovum Interactions chemistry.chemical_classification Serine protease Mesocricetus biology General Medicine biology.organism_classification Sperm Molecular biology Peptide Fragments chemistry Immunology Oocytes biology.protein Female Protein C medicine.drug |
| Zdroj: | Andrologia. 31:217-223 |
| ISSN: | 1439-0272 0303-4569 |
| DOI: | 10.1046/j.1439-0272.1999.00282.x |
| Popis: | Protein C inhibitor is a heparin-dependent serine protease inhibitor present in plasma at about 0.08 mumol l-1. Protein C inhibitor inhibits activated protein C and other coagulation factors. Previously, we described the presence of high protein C inhibitor levels in human semen (3.1 mumol l-1) and showed potential roles of the inhibitor in human reproduction. Here, we show that protein C inhibitor is present in an active form in follicular fluid at about 0.1 mumol l-1 and that purified, functionally active human plasma-derived and inactive, semen-derived protein C inhibitor and a synthetic peptide derived from its sequence inhibited both binding and penetration of zona-free hamster oocytes by human sperm. The binding inhibition by protein C inhibitor was dose dependent, with 50% inhibition at 0.037 mumol l-1 inhibitor (45 +/- 17 sperm per egg versus 90 +/- 23 in control experiments). The inhibitor also blocked in a dose-dependent manner the penetration of zona-free hamster eggs by human sperm (20 +/- 7% fertilized eggs at 0.1 mumol l-1 protein C inhibitor versus 55 +/- 10% in control experiments). Polyclonal antiprotein C inhibitor or antipeptide antibodies partially abolished the effect of protein C inhibitor and peptide on the inhibition of the binding and penetration of zona-free hamster oocytes by human sperm. The effect of the protein C inhibitor was not dependent on its antiprotease activity since purified semen-derived protein C inhibitor which did not have antiprotease activity gave comparable results. We conclude that protein C inhibitor may be involved in human reproduction at several steps, including the fertilization process. |
| Databáze: | OpenAIRE |
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