Quorum sensing in Enterococcus faecium, Enterococcus faecalis and Bacillus cereus strains isolated from ricotta processing
| Autor: | Dirce Yorika Kabuki, Arnaldo Yoshiteru Kuaye, Luciana Maria Ramires Esper, Meg da Silva Fernandes |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
030106 microbiology Bacillus cereus Enterococcus faecalis Microbiology lcsh:Agriculture 03 medical and health sciences lcsh:Agriculture (General) biology Vibrio harveyi Chemistry Enterococcus spp fungi AI-2 lcsh:S luxS biochemical phenomena metabolism and nutrition biology.organism_classification lcsh:S1-972 bioluminescence Quorum sensing 030104 developmental biology Enterococcus Cereus bacteria Autoinducer Enterococcus faecium |
| Zdroj: | Ciência Rural v.48 n.2 2018 Ciência Rural Universidade Federal de Santa Maria (UFSM) instacron:UFSM Ciência Rural, Vol 48, Iss 2 (2018) |
| Popis: | portuguesO fenomeno quorum sensing corresponde a um processo de comunicacao intra e interespecies microbianas e e mediado por sinais quimicos extracelulares, denominados moleculas sinalizadoras ou auto indutoras (AI). A molecula AI2 esta envolvida na comunicacao interespecies, denominada sistema “universal” de comunicacao. Este estudo teve como objetivo avaliar a capacidade de Enterococcus faecium, Enterococcus faecalis e Bacillus cereus isolados do processamento de ricota em produzir moleculas sinalizadoras de Quorum sensing (AI-2). Os isolados foram avaliados quanto a presenca do gene luxS utilizando a reacao em cadeia da polimerase (PCR). As moleculas sinalizadoras (AI-2) foram medidas em unidades relativas de luz (RLU) atraves de um luminometro. Um total de 74% dos isolados de E. faecium, 91% de E. faecalis e 95% de B. cereus foram positivos para o gene luxS. Alem disso, todos os isolados apresentaram capacidade de induzir o fenomeno de bioluminescencia em Vibrio harveyi BB170, indicando a presenca de auto indutores AI-2. Palavras-chave: luxS; AI-2; Vibrio harveyi; bioluminescencia; Enterococcus spp.; Bacillus cereus EnglishThe quorum sensing phenomenon is a process of intra- and inter-species microbial communication involving the production and detection of extracellular signaling molecules. The autoinducer AI-2 has been proposed to serve as a ‘universal signal’ for interspecies communication. This study aimed to evaluate the capability of Enterococcus faecium, Enterococcus faecalis, and Bacillus cereus strains isolated from ricotta processing to produce quorum sensing signalling molecules (AI-2). The strains were evaluated for the presence of the luxS gene using the polymerase chain reaction. AI-2 quorum sensing signalling molecules were measured in relative light units (RLUs) using a luminometer. A total of 74% of E. faecium, 91% of E. faecalis, and 95% of B. cereus isolates were positive for luxS gene. In addition, the induced bioluminescence in Vibrio harveyi BB170 was observed in all strains, indicating the presence of the AI-2 autoinducer. Key words: luxS; AI-2; Vibrio harveyi; bioluminescence; Enterococcus spp.; Bacillus cereus |
| Databáze: | OpenAIRE |
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