Expression of High Molecular Weight Cysteine Proteinase Inhibitor in Ovarian Cancer Tissues: Regulation of Cathepsin B Expression by Placental CPI
| Autor: | Yousif Saleh, Maciej Siewinski, Michał Jeleń, Piotr Ziółkowski, Andrzej Popiela, Marian Gryboś |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Adult
endocrine system diseases Placenta Clinical Biochemistry Gene Expression Cysteine Proteinase Inhibitors Biochemistry Antibodies Cathepsin B Metastasis medicine Humans Molecular Biology Ovarian Neoplasms Cathepsin Antiserum Enzyme Precursors biology Tissue Extracts Middle Aged medicine.disease Immunohistochemistry Molecular biology female genital diseases and pregnancy complications Molecular Weight Polyclonal antibodies biology.protein Female Antibody Ovarian cancer |
| Zdroj: | Biological Chemistry. 384 |
| ISSN: | 1431-6730 |
| DOI: | 10.1515/bc.2003.123 |
| Popis: | Mouse polyclonal antibodies against placental cysteine proteinase inhibitor (CPI) react with the placental 67 kDa CPI on Western blots, and CPI present in ovarian cancer homogenate and serum was shown by double immunodiffusion to react with the same antiserum. By immunohistochemical staining, positive expression of high molecular weight CPI was observed on the tumour cell surface in serous and endometrioid ovarian carcinomas with metastasis. Normal endometrioid tissue was not stained with anti-placental CPI antibodies. Cathepsin B and pro-cathepsin B median levels in ovarian cancer tissue homogenates increased progressively with FIGO stage of the disease. The enzyme level decreased 22-fold after treatment of tissue homogenates with 5 nM purified CPI. These results provide evidence that addition of CPI reduces the levels of cysteine-type cathepsins to those of normal non-cancerous values. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|