Expansion of the NKG2C+ natural killer-cell subset is associated with high-risk carotid atherosclerotic plaques in seropositive patients for human cytomegalovirus
| Autor: | Angel Ois, Luis Roig, Elisa Cuadrado, Jaume Roquer, Francesc Alameda, Jose E Martínez-Rodríguez, Jessica Munné-Collado, Miguel López-Botet, Aura Muntasell, Teresa Baró, Raquel Rasal |
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| Rok vydání: | 2013 |
| Předmět: |
Human cytomegalovirus
Carotid Artery Diseases Male Pathology medicine.medical_specialty CD3 Cytomegalovirus Biology Systemic inflammation Antibodies Viral Natural killer cell Immunophenotyping Risk Factors Seroepidemiologic Studies medicine Humans Receptor Aged Aged 80 and over Middle Aged medicine.disease CD56 Antigen Plaque Atherosclerotic Killer Cells Natural medicine.anatomical_structure Immunology Cytomegalovirus Infections biology.protein Female medicine.symptom Cardiology and Cardiovascular Medicine NK Cell Lectin-Like Receptor Subfamily C CD8 |
| Zdroj: | Arteriosclerosis, thrombosis, and vascular biology. 33(11) |
| ISSN: | 1524-4636 |
| Popis: | Objective— Human cytomegalovirus (HCMV), a pathogen involved in the development and progression of atherosclerosis, promotes in some individuals a marked reconfiguration of the natural killer (NK)–cell compartment whose hallmark is a persistent expansion of a peripheral blood NK-cell subset expressing the CD94/NKG2C NK receptor. We aimed to evaluate whether the HCMV-associated NK-cell compartment reconfiguration is related to carotid atherosclerotic plaque (CAP) instability. Approach and Results— NK receptor expression (ie, LILRB1, NKG2A, NKG2C, and killer immunoglobulin-like receptors [KIR]) by peripheral NK and T cells was evaluated in 40 patients with HCMV+ with CAP, including nonatherosclerotic strokes (n=15) and healthy subjects (n=11) as controls. High-risk CAP (n=16), defined as carotid stenosis >50% with ipsilateral neurological symptomatology in the previous 180 days, compared with non–high-risk CAP had higher %NKG2C+ NK cells (29.5±22.4% versus 16.3±13.2%; P =0.026; odds ratio, 1.053; 95% confidence interval, 1.002–1.106; P =0.042), with a corresponding reduction in the NKG2A+ NK subset (31.7±17.8% versus 41.8±15.8%; P =0.072). The proportions of NKG2C+ NK cells in high-risk CAP were inversely correlated with the CD4+/CD8+ ratio ( R Spearman =−0.629; P =0.009) and directly with high-sensitivity C-reactive protein levels ( R Pearson =0.591; P =0.012), consistent with higher subclinical systemic inflammation. The intraplaque inflammatory infiltrate, evaluated in 27 CAP obtained after endarterectomy, showed a higher presence of subintimal CD3+ lymphocytes in those patients with HCMV-induced changes in the peripheral NK- and T-cell compartments. Conclusions— The expansion of NKG2C+ NK cells in patients with CAP seems to be associated with an increased risk of plaque destabilization in some patients with chronic HCMV infection. |
| Databáze: | OpenAIRE |
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