Influenza A Virus Induces Autophagosomal Targeting of Ribosomal Proteins
| Autor: | Jörn Dengjel, Verónica I. Dumit, Lea Bühler, Carole Roubaty, Martin Schwemmle, Shadi Abou-Eid, Christine Gretzmeier, Monique Gannagé, Zehan Hu, Stéphanie Kaeser-Pebernard, Andrea C. Becker, Christian Münz, Sebastian Giese, Petra Paul |
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| Přispěvatelé: | University of Zurich, Dengjel, Jörn |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
1303 Biochemistry Proteome organelle Cell autophagosome medicine.disease_cause Proteomics 10263 Institute of Experimental Immunology SILAC Autophagosomes/*metabolism Autophagy Cell Line Tumor Humans Influenza A virus/*metabolism Influenza Human/metabolism/pathology/virology Proteome/metabolism RNA Messenger/genetics/metabolism RNA Viral/metabolism Ribosomal Proteins/*metabolism Ribosomes/metabolism Cell biology Ribosomes silac Viruses cell line proteomics vesicle Biochemistry Analytical Chemistry 0302 clinical medicine Interferon Influenza A virus 1602 Analytical Chemistry 3. Good health medicine.anatomical_structure RNA Viral medicine.drug Ribosomal Proteins Viral protein 610 Medicine & health Biology Virus 03 medical and health sciences Ribosomal protein Influenza Human medicine 1312 Molecular Biology RNA Messenger Molecular Biology Research Autophagosomes Virology 030104 developmental biology Cell culture 570 Life sciences biology 030217 neurology & neurosurgery |
| Zdroj: | Mol Cell Proteomics, vol. 17, no. 10, pp. 1909-1921 Molecular & Cellular Proteomics : MCP |
| Popis: | Seasonal epidemics of influenza A virus are a major cause of severe illness and are of high socio-economic relevance. For the design of effective antiviral therapies, a detailed knowledge of pathways perturbed by virus infection is critical. We performed comprehensive expression and organellar proteomics experiments to study the cellular consequences of influenza A virus infection using three human epithelial cell lines derived from human lung carcinomas: A549, Calu-1 and NCI-H1299. As a common response, the type I interferon pathway was up-regulated upon infection. Interestingly, influenza A virus infection led to numerous cell line-specific responses affecting both protein abundance as well as subcellular localization. In A549 cells, the vesicular compartment appeared expanded after virus infection. The composition of autophagsomes was altered by targeting of ribosomes, viral mRNA and proteins to these double membrane vesicles. Thus, autophagy may support viral protein translation by promoting the clustering of the respective molecular machinery in autophagosomes in a cell line-dependent manner. |
| Databáze: | OpenAIRE |
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