SALT-RESPONSIVE ERF1 Regulates Reactive Oxygen Species–Dependent Signaling during the Initial Response to Salt Stress in Rice[W]
| Autor: | Jos H. M. Schippers, Emmanuel Guiderdoni, Romy Schmidt, Hans-Michael Hubberten, Alisdair R. Fernie, Bernd Mueller-Roeber, Toshihiro Obata, Blanca San Segundo, Delphine Mieulet, Rainer Hoefgen, Joachim Fisahn |
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| Přispěvatelé: | European Research Council, Federal Ministry of Science, Research and Economy (Germany), Agence Nationale de la Recherche (France), Ministerio de Educación y Ciencia (España), Agropolis Fondation, European Commission |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
MAPK/ERK pathway
Résistance génétique Plant Science MAPK cascade Sodium Chloride F30 - Génétique et amélioration des plantes Salinité Gene Expression Regulation Plant Tolérance au sel Expression des gènes Promoter Regions Genetic Research Articles Plant Proteins Zinc finger chemistry.chemical_classification Regulation of gene expression Microscopy Confocal Reverse Transcriptase Polymerase Chain Reaction food and beverages Gene Expression Regulation Developmental Salt Tolerance MAP Kinase Kinase Kinases Oxidants Plants Genetically Modified dérivé réactif de l'oxygène Cell biology Biochemistry Sel Gene Knockdown Techniques Protein Binding Signal Transduction F60 - Physiologie et biochimie végétale Molecular Sequence Data Transformation génétique Oryza sativa Biology Stress Physiological Péroxyde Protein kinase A Transcription factor Institut für Biochemie und Biologie Reactive oxygen species Models Genetic Wild type Oryza Cell Biology Hydrogen Peroxide Plante transgénique Stress osmotique Luminescent Proteins chemistry Mutation H50 - Troubles divers des plantes Reactive Oxygen Species Transcription Factors |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Plant Cell |
| Popis: | Early detection of salt stress is vital for plant survival and growth. Still, the molecular processes controlling early salt stress perception and signaling are not fully understood. Here, we identified SALT-RESPONSIVE ERF1 (SERF1), a rice (Oryza sativa) transcription factor (TF) gene that shows a root-specific induction upon salt and hydrogen peroxide (H2O2) treatment. Loss of SERF1 impairs the salt-inducible expression of genes encoding members of a mitogen-activated protein kinase (MAPK) cascade and salt tolerance–mediating TFs. Furthermore, we show that SERF1-dependent genes are H2O2 responsive and demonstrate that SERF1 binds to the promoters of MAPK KINASE KINASE6 (MAP3K6), MAPK5, DEHYDRATION-RESPONSIVE ELEMENT BINDING2A (DREB2A), and ZINC FINGER PROTEIN179 (ZFP179) in vitro and in vivo. SERF1 also directly induces its own gene expression. In addition, SERF1 is a phosphorylation target of MAPK5, resulting in enhanced transcriptional activity of SERF1 toward its direct target genes. In agreement, plants deficient for SERF1 are more sensitive to salt stress compared with the wild type, while constitutive overexpression of SERF1 improves salinity tolerance. We propose that SERF1 amplifies the reactive oxygen species–activated MAPK cascade signal during the initial phase of salt stress and translates the salt-induced signal into an appropriate expressional response resulting in salt tolerance. This work was in part conducted within the European Reseacrh Area Network Plant Genomics project ‘TRIESTER - Trilateral Initiative for Enhancing Salt Tolerance in Rice’ for which funding was provided by the Bundesministerium für Bildung und Forschung (BMBF) in Germany (Grant 0313993A), the Agence Nationale de la Recherche in France (Grant ANR-06-ERAPG-005-01), and Ministerio de Educación y Ciencia (Grant GEN2006-27794-C4-1-E) in Spain. Part of this work was conducted on the Rice Functional Genomics platform, Montpellier, France, funded by Agropolis Foundation. R.S. thanks the FAZIT Stiftung for providing a PhD fellowship. |
| Databáze: | OpenAIRE |
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