Phylogenetic grouping and pathotypic comparison of urine and fecal Escherichia coli isolates from children with urinary tract infection
| Autor: | Bita Bakhshi, Masoumeh Navidinia, Fatemeh Fallah, Raheleh Sadat Sajadi-Nia, Shahin Najar Peerayeh |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
Cefotaxime Nalidixic acid lcsh:QR1-502 Urine urologic and male genital diseases Integron medicine.disease_cause Cefpodoxime Polymerase Chain Reaction lcsh:Microbiology Feces Hemolysin Proteins Genotype Cluster Analysis Child Urinary tract infection (UTI) Escherichia coli Infections Phylogeny biology Escherichia coli Proteins intI1 Hemolysin QR1-502 intll Anti-Bacterial Agents Medical Microbiology phylogenetic typing groups Child Preschool Urinary Tract Infections Female Research Paper medicine.drug Microbiology Drug Resistance Bacterial Escherichia coli medicine Humans Integrases Genetic Variation Membrane Transport Proteins Sequence Analysis DNA bacterial infections and mycoses medicine.disease urinary tract infection (UTI) biology.protein hlyD |
| Zdroj: | Brazilian Journal of Microbiology Brazilian Journal of Microbiology v.45 n.2 2014 Sociedade Brasileira de Microbiologia (SBM) instacron:SBM Brazilian Journal of Microbiology, Vol 45, Iss 2, Pp 509-514 (2014) Brazilian Journal of Microbiology, Volume: 45, Issue: 2, Pages: 509-514, Published: JUN 2014 |
| ISSN: | 1517-8382 |
| DOI: | 10.1590/s1517-83822014000200019 |
| Popis: | The aim of this study was to investigate the phylogenetic background and to assess hlyD (involved in the secretion of haemolysin A) and intll (encoding a class 1 integrase) in Escherichia coli isolates derived from urinary and fecal specimens. A total of 200 E. coli isolates was collected from patients presenting with urinary tract infection (UTI) during September 2009 to September 2010 and screened for hlyD and intll genes by polymerase chain reaction (PCR). Phylogenetic analysis showed that E. coli is composed of four main phylogenetic groups (A, B1, B2 and D) and that uropathogenic E. coli (UPEC) isolates mainly belong to groups B2 (54%) and D (34%) whereas group A (44%) and D (26%) are predominant among commensal E. coli isolates. In this study, hlyD was present in 26% of UPEC and 2% of commensal E. coli isolates. However, hemolytic activity was detected for 42% of UPEC and 6% of commensal E. coli isolates (p < 0.05). intll gene was more frequently expressed in UPEC (24%) in comparison with commensal E. coli isolates (12%). Resistance to aztreonam, co-trimoxazole and cefpodoxime were frequently found among UPEC isolates whereas commensal E. coli isolates were commonly resistant to co-trimoxazole, nalidixic acid and cefotaxime. Concluding, a considerable difference between UPEC and commensal E. coli isolates was observed regarding their phylogenetic groups, presence of class 1 integron and hlyD gene, hemolysin activity and resistance pattern. The detection of class 1 integrons and hlyD gene was higher among UPEC compared with commensal E. coli isolates. These findings may contribute for a better understanding of the factors involved in the pathogenesis of UPEC. |
| Databáze: | OpenAIRE |
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