Portable, low cost and sensitive cavity enhanced absorption (CEA) detection
| Autor: | Andrew Teggert, Zulfiqur Ali, Barry Warden, Stephen McIntosh, Simon Bateson, Fathi Abugchem, Harish K. Datta |
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| Rok vydání: | 2021 |
| Předmět: |
Materials science
Optical fiber 02 engineering and technology 01 natural sciences Biochemistry Analytical Chemistry law.invention chemistry.chemical_compound law Wide dynamic range Electrochemistry Rhodamine B Environmental Chemistry Spectroscopy Spectrometer business.industry 010401 analytical chemistry 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences Photodiode chemistry Optical cavity Optoelectronics 0210 nano-technology business Light-emitting diode |
| Zdroj: | The Analyst. 146:196-206 |
| ISSN: | 1364-5528 0003-2654 |
| DOI: | 10.1039/d0an01852j |
| Popis: | Absorption is a widely used technique for a range of different applications. It has lower sensitivity than many other techniques such as fluorescence which has 100 to 1000 times higher sensitivity than absorption. Optical cavity approaches have been developed where the light passes back and forth, within the sample, between two high reflectivity mirrors to increase the pathlength and sensitivity. These approaches have not yet, however, been widely used for analytical applications and for point-of-care diagnostics. Here we show a portable cavity enhanced absorption (CEA) spectrometer and a low cost point-of-care (POC) reader with CEA detection with mechanical elements fabricated using 3D printing. The CEA spectrometer can be used in both single pass and multi-pass cavity enhanced mode to provide measurements in the visible region that are very sensitive and over a wide dynamic range. The CEA mode was shown for Rhodamine B dye to increase the pathlength 57.8 fold over single pass measurements and an LOD of 7.1 × 10-11 M. The cost of the CEA POC reader was reduced by use of narrow band LEDs, photodiodes and removal of fibre optic coupling and with a 14 fold increase in the pathlength over conventional single pass microplate readers. The CEA POC reader was demonstrated for immunoassay of C-Reactive Protein (CRP), Procalcitonin (PCT) and Interleukin 6 (IL-6), towards a three biomarker panel to aid the diagnosis of sepsis. The CEA POC reader can be integrated with wireless connectivity for cloud based data sharing. We show here the potential for the wider use of optical cavity approaches where there is a need for sensitive absorption measurements and also for low cost point-of-care diagnostics. |
| Databáze: | OpenAIRE |
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