Vaccination with autologous tumor-loaded dendritic cells induces clinical and immunologic responses in indolent B-cell lymphoma patients with relapsed and measurable disease: a pilot study
Autor: | Paola Baldassari, Roberta Mortarini, Carmelo Carlo-Stella, Michele Magni, Liliana Devizzi, Roberta Zappasodi, Alessandro M. Gianni, Massimo Di Nicola, Paola Matteucci, Andrea Anichini, Fernando Ravagnani, Antonello Cabras, Serenella M. Pupa |
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Rok vydání: | 2008 |
Předmět: |
Male
Quality Control Lymphoma B-Cell Immunology Pilot Projects Biochemistry Cancer Vaccines Immunotherapy Adoptive T-Lymphocytes Regulatory Immunophenotyping Recurrence medicine Humans IL-2 receptor B-cell lymphoma Aged business.industry FOXP3 Cell Biology Hematology Dendritic Cells Middle Aged medicine.disease Lymphoma Vaccination Killer Cells Natural Measurable Disease Treatment Outcome Immunohistochemistry Female business Tomography X-Ray Computed Progressive disease Follow-Up Studies |
Zdroj: | Blood. 113(1) |
ISSN: | 1528-0020 |
Popis: | Eighteen relapsed patients with measurable indolent non-Hodgkin lymphoma (NHL) were vaccinated with dendritic cells (DCs) loaded with killed autologous tumor cells. Six patients had objective clinical responses including 3 continuous complete responses (CRs) and 3 partial responses (PRs), with a median follow up of 50.5 months. Eight patients had stable disease, whereas 4 had progressive disease. Clinical responses were significantly associated with a reduction in CD4+CD25+FOXP3+ regulatory T cells, an increase in CD3−CD56dimCD16+ natural killer (NK) cells, and maturation of lymphocytes to the effector memory stage in either postvaccination peripheral blood or tumor specimen samples. In partial responding patients, vaccination significantly boosted the IFN-γ–producing T-cell response to autologous tumor challenge. In one HLA-A*0201+ patient who achieved CR, IL-4 release by circulating T cells in response to tumor-specific IgH-encoded peptides was also documented. Immunohistochemical analysis of tumor biopsies using biotin-conjugated autologous serum samples revealed a tumor-restricted humoral response only in the postvaccination serum from responding patients. Collectively these results demonstrate that vaccination with tumor-loaded DCs may induce both T- and B-cell responses and produces clinical benefits in indolent NHL patients with measurable disease. This study is registered with the Istituto Superiore di Sanità: http://www.iss.it with protocol number 7578-PRE 21-801. |
Databáze: | OpenAIRE |
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