Characterization of bacterial diversity in contaminated groundwater using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
| Autor: | Inês C. Santos, Kevin A. Schug, Misty S. Martin, Zacariah L. Hildenbrand, Doug D. Carlton, Paula E Stigler-Granados |
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| Rok vydání: | 2018 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Environmental Engineering BTEX 010501 environmental sciences medicine.disease_cause 01 natural sciences 03 medical and health sciences Microbial ecology RNA Ribosomal 16S medicine Environmental Chemistry Groundwater Waste Management and Disposal 0105 earth and related environmental sciences Bacteria biology Chemistry Drinking Water Pathogenic bacteria Contamination biology.organism_classification Pollution Stenotrophomonas maltophilia 030104 developmental biology Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Environmental chemistry Water treatment |
| Zdroj: | Science of The Total Environment. :1562-1571 |
| ISSN: | 0048-9697 |
| DOI: | 10.1016/j.scitotenv.2017.10.027 |
| Popis: | Groundwater is a major source for drinking water in the United States, and therefore, its quality and quantity is of extreme importance. One major concern that has emerged is the possible contamination of groundwater due to the unconventional oil and gas extraction activities. As such, the impacts of exogenous contaminants on microbial ecology is an area to be explored to understand what are the chemical and physical conditions that allow the proliferation of pathogenic bacteria and to find alternatives for water treatment by identifying organic-degrading bacteria. In this work, we assess the interplay between groundwater quality and the microbiome in contaminated groundwaters rich in hydrocarbon gases, volatile organic and inorganic compounds, and various metals. Opportunistic pathogenic bacteria, such as Aeromonas hydrophila, Bacillus cereus, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia, were identified, increasing the risk for consumption of and exposure to these contaminated groundwaters. Additionally, antimicrobial tests revealed that many of the identified bacteria were resistant to different antibiotics. The MALDI-TOF MS results were successfully confirmed with 16S rRNA gene sequencing, proving the accuracy of this high-throughput method. Collectively, these data provide a seminal understanding of the microbial populations in contaminated groundwater overlying anthropogenic activities like unconventional oil and gas development. |
| Databáze: | OpenAIRE |
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