Simultaneous comprehensive multiplex autoantibody analysis for rapidly progressive glomerulonephritis
| Autor: | Peter Schierack, Mandy Sowa, Barbara Trezzi, Renato Alberto Sinico, Kai Grossmann, Valentina Somma, Antonella Radice, Juliane Scholz, Dirk Roggenbuck, Rico Hiemann |
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| Přispěvatelé: | Sowa, M, Trezzi, B, Hiemann, R, Schierack, P, Grossmann, K, Scholz, J, Somma, V, Sinico, R, Roggenbuck, D, Radice, A |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Pathology medicine.medical_specialty Time Factors Neutrophils urologic and male genital diseases Diagnostic Accuracy Study Antibodies Antineutrophil Cytoplasmic 03 medical and health sciences Rapidly progressive glomerulonephriti 0302 clinical medicine Glomerulonephritis Proteinase 3 immune system diseases Medicine Goodpasture syndrome Rapidly progressive glomerulonephritis Humans cardiovascular diseases skin and connective tissue diseases Fluorescent Antibody Technique Indirect rapidly progressive glomerulonephritis Digital fluorescence Microbead Anti-neutrophil cytoplasmic antibody Aged 030203 arthritis & rheumatology Immunoassay business.industry Medicine (all) Autoantibody IIf General Medicine Middle Aged medicine.disease 030104 developmental biology Case-Control Studies Child Preschool Immunology Disease Progression Female business Microscopic polyangiitis Granulomatosis with polyangiitis Research Article |
| Zdroj: | Medicine |
| Popis: | Rapidly progressive glomerulonephritis (RPGN) is mainly caused by anti-glomerular basement membrane (GBM) antibody-mediated glomerulonephritis, immune-complex or anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitides and leads to rapid loss of renal function. Detection of ANCA and autoantibodies (autoAbs) to GBM and dsDNA enables early diagnosis and appropriate treatment of RPGN aiding in preventing end-stage renal disease. Determination of ANCA on neutrophils (ANCA) as well as autoAbs to myeloperoxidase (MPO-ANCA), proteinase 3 (PR3-ANCA), GBM, and dsDNA was performed by the novel multiplex CytoBead technology combining cell- and microbead-based autoAb analyses by automated indirect immunofluorescence (IIF). Forty patients with granulomatosis with polyangiitis (GPA), 48 with microscopic polyangiitis (MPA), 2 with eosinophilic GPA, 42 with systemic lupus erythematosus (SLE), 43 with Goodpasture syndrome (GPS), 57 with infectious diseases (INF), and 55 healthy subjects (HS) were analyzed and findings compared with classical single testing. The CytoBead assay revealed for GPA, MPA, GPS, and SLE the following diagnostic sensitivities and for HS and INF the corresponding specificities: PR3-ANCA, 85.0% and 100.0%; MPO-ANCA, 77.1% and 99.1%; anti-GBM autoAb, 88.4% and 96.4%; anti-dsDNA autoAb, 83.3% and 97.3%; ANCA, 91.1% and 99.1%, respectively. Agreement with classical enzyme-linked immunosorbent assay and IIF was very good for anti-GBM autoAb, MPO-ANCA, PR3-ANCA, and ANCA, respectively. Anti-dsDNA autoAb comparative analysis demonstrated fair agreement only and a significant difference (P = 0.0001). The CytoBead technology provides a unique multiplex reaction environment for simultaneous RPGN-specific autoAb testing. CytoBead RPGN assay is a promising alternative to time-consuming single parameter analysis and, thus, is well suited for emergency situations. |
| Databáze: | OpenAIRE |
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