Tubulin Acetylation Mediates Bisphenol A Effects on the Microtubule Arrays of Allium cepa and Triticum turgidum
| Autor: | Emmanuel Panteris, Ioannis-Dimosthenis S. Adamakis, Eleftherios P. Eleftheriou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
endocrine system
Paclitaxel Protein subunit bisphenol A lcsh:QR1-502 cytokinesis 010501 environmental sciences Hydroxamic Acids 01 natural sciences Biochemistry Article lcsh:Microbiology microtubules 03 medical and health sciences Phenols Tubulin Microtubule Onions Benzhydryl Compounds Molecular Biology Mitosis Triticum Plant Proteins 030304 developmental biology 0105 earth and related environmental sciences mitosis 0303 health sciences biology Chemistry urogenital system food and beverages Acetylation Tubulin Modulators 3. Good health Cell biology biology.protein tubulin acetylation Interphase Protein Processing Post-Translational Cortical microtubule Cytokinesis hormones hormone substitutes and hormone antagonists |
| Zdroj: | Biomolecules, Vol 9, Iss 5, p 185 (2019) Biomolecules Volume 9 Issue 5 |
| Popis: | The effects of bisphenol A (BPA), a prevalent endocrine disruptor, on both interphase and mitotic microtubule array organization was examined by immunofluorescence microscopy in meristematic root cells of Triticum turgidum (durum wheat) and Allium cepa (onion). In interphase cells of A. cepa, BPA treatment resulted in substitution of cortical microtubules by annular/spiral tubulin structures, while in T. turgidum BPA induced cortical microtubule fragmentation. Immunolocalization of acetylated &alpha tubulin revealed that cortical microtubules of T. turgidum were highly acetylated, unlike those of A. cepa. In addition, elevation of tubulin acetylation by trichostatin A in A. cepa resulted in microtubule disruption similar to that observed in T. turgidum. BPA also disrupted all mitotic microtubule arrays in both species. It is also worth noting that mitotic microtubule arrays were acetylated in both plants. As assessed by BPA removal, its effects are reversible. Furthermore, taxol-stabilized microtubules were resistant to BPA, while recovery from oryzalin treatment in BPA solution resulted in the formation of ring-like tubulin conformations. Overall, these findings indicate the following: (1) BPA affects plant mitosis/cytokinesis by disrupting microtubule organization. (2) Microtubule disassembly probably results from impairment of free tubulin subunit polymerization. (3) The differences in cortical microtubule responses to BPA among the species studied are correlated to the degree of tubulin acetylation. |
| Databáze: | OpenAIRE |
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