Slow and ultra-rapid freezing protocols for cryopreserving roe deer (Capreolus capreolus) epididymal sperm collected at different times of year

Autor: Lucía Martínez-Fresneda, Paula Bóveda, Antonio López-Sebastián, Julián Santiago-Moreno, J. L Marcos-Beltrán, Rosario Velázquez, V.N. Flores-Gil, Milagros C. Esteso, Cristina Castaño, O. Mejía, A. M. González-Guirado, Adolfo Toledano-Díaz
Přispěvatelé: Ministerio de Economía y Competitividad (España), Agencia Estatal de Investigación (España), European Commission, Universidad Nacional Autónoma de México, Fondo Nacional de Desarrollo Científico, Tecnológico y de Información Tecnológica (Perú), Santiago-Moreno, J., Castaño, C., Bóveda, P., Martínez-Fresneda, L., Flores-Gil, V. N., Toledano-Díaz, A, López-Sebastián, A.
Rok vydání: 2021
Předmět:
Zdroj: European Journal of Wildlife Research. 67
ISSN: 1439-0574
1612-4642
DOI: 10.1007/s10344-021-01468-4
Popis: 10 Pág. Centro de Investigación en Sanidad Animal (CISA) / Departamento de Reproducción animal
The roe deer is a monoestrous species with a very short rutting season. The present work reports the most suitable period for collecting epididymal sperm and describes the effect of two cooling rates on the post-thaw quality of sperm. Testes were collected 24–48 h after death. Samples of sperm flushed from the epididymis were subjected to either (1) dilution in a Tris-citric acid-glucose-egg yolk-based medium with glycerol, and slow freezing in straws, or (2) dilution in the same extender but replacing the glycerol with 100 mM of sucrose, and ultra-rapid freezing in pellets. Sperm motility, acrosome and membrane integrity, morphometry and morphological abnormalities were analysed before and after cryopreservation. Spermatogenic activity was investigated via histological examination of testis sections. Several testes collected between April, May and September showed no spermatogenic activity. All those collected in June–August showed spermatogenic activity. No significant difference was detected in the cryoresistance ratios associated with the conventional slow freezing, between sperm collected during the pre-rutting (April–May) and rutting (June–August) periods. No significant differences were seen between the slow-frozen-thawed and the ultra-rapid-frozen-thawed sperm in terms of percentage of viable sperm or the percentage of sperm with morphological abnormalities. Slow freezing returned significantly better (P
This research was funded by MINECO/AEI/FEDER and EU grant AGL2017-85753-R. P. Bóveda was the recipient of a grant for pre-doctoral researchers from MINECO (AEI/FSE, UE). Octavio Mejía was the recipient of a research fellowship from the PASPA-DGAPA-UNAM (México). V.N. Flores-Gil was funded by FONDECYT-CONCYTEC (grant contract number 000245-2015-FONDECYT).
Databáze: OpenAIRE
Externí odkaz: