Nectar-dwelling microbes of common tansy are attractive to its mosquito pollinator,Culex pipiensL
| Autor: | Peach, D. A. H., Carroll, C., Meraj, S., Gomes, S., Galloway, E., Balcita, A., Coatsworth, H., Young, N., Uriel, Y., Gries, R., Lowenberger, C., Moore, M., Gries, G. |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
2. Zero hunger 0303 health sciences biology Pollination Microorganism media_common.quotation_subject fungi food and beverages Insect biology.organism_classification 010603 evolutionary biology 01 natural sciences 03 medical and health sciences Odor Pollinator Botany Culex pipiens Nectar Micrococcus luteus 030304 developmental biology media_common |
| DOI: | 10.1101/2020.04.03.024380 |
| Popis: | BackgroundThere is widespread interkingdom signalling between insects and microbes. For example, microbes found in floral nectar may modify its nutritional composition and produce odorants that alter the floral odor bouquet which may attract insect pollinators. Mosquitoes consume nectar and can pollinate flowers. We identified microbes isolated from nectar of common tansy,Tanacetum vulgare,elucidated the microbial odorants, and tested their ability to attract the common house mosquito,Culex pipiens.ResultsWe collected 18 microbial isolates fromT. vulgarenectar, representing at least 12 different taxa which we identified with 16S or 26S rDNA sequencing as well as by biochemical and physiological tests. Three microorganisms(Lachancea thermotolerans, Micrococcus lactis, Micrococcus luteus)were grown on culture medium and tested in bioassays. Only the yeastL. thermotoleransgrown on nectar, malt extract agar, or in synthetic nectar broth significantly attractedC. pipiensfemales. The odorant profile produced byL. thermotoleransvaried with the nutritional composition of the culture medium. Surprisingly, all three microbes grown separately, but presented concurrently, attracted fewerC. pipiensfemales thanL. thermotoleransby itself.ConclusionsFloral nectar ofT. vulgarecontains various microbes whose odorants contribute to the odor profile of inflorescences. In addition,L. thermotoleransproduced odorants that attractCx. pipiensfemales. As the odor profile ofL. thermotoleransvaried with the composition of the culture medium, we hypothesize that microbe odorants inform nectar-foraging mosquitoes about the availability of certain macro-nutrients which, in turn, affect foraging decisions by mosquitoes. |
| Databáze: | OpenAIRE |
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