Apoptosis in human unfertilized oocytes after intracytoplasmic sperm injection
| Autor: | Maurizio Manno, Liana Bosco, Giovanni Ruvolo, Maria Carmela Roccheri, Giovanni Morici, Ettore Cittadini |
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| Přispěvatelé: | BOSCO, L, RUVOLO, G, MORICI, G, MANNO, M, CITTADINI, E, ROCCHERI, MC |
| Rok vydání: | 2005 |
| Předmět: |
virgin oocytes
medicine.medical_treatment intracytoplasmic sperm injection Apoptosis DNA Fragmentation Biology ICSI Intracytoplasmic sperm injection Andrology Human fertilization human oocyte Confidence Intervals medicine Humans Sperm Injections Intracytoplasmic reproductive and urinary physiology Fertilisation fertilization failure human oocytes Chi-Square Distribution In vitro fertilisation TUNEL assay urogenital system Apoptosi Obstetrics and Gynecology Oocyte Sperm medicine.anatomical_structure Reproductive Medicine Oocytes DNA fragmentation Female |
| Zdroj: | Fertility and Sterility. 84:1417-1423 |
| ISSN: | 0015-0282 |
| Popis: | Objective To investigate the presence of programmed cell death in unfertilized oocytes after intracytoplasmic sperm injection (ICSI), assuming that previous apoptotic events could be correlated with the fertilization failure. Design Comparison of the rate of DNA fragmentation in human oocytes at different stages of maturation soon after pick-up (control) and in unfertilized oocytes after ICSI treatment. Setting In vitro fertilization (IVF) laboratory with extensive ICSI experience. Patient(s) Sixty-three patients undergoing assisted fertilization by ICSI. Intervention(s) Terminal deoxynucleotidyl transferase-mediated digoxigenin-dUTP nick-end labeling (TUNEL) assay and anticaspase-3 cleaved immunoassay to detect apoptosis in control and ICSI-treated oocytes. Main Outcome Measure(s) Differences in the percentage of oocytes demonstrating DNA fragmentation between control oocytes and unfertilized ICSI treated oocytes at different stages of maturation. Result(s) The DNA fragmentation, by TUNEL assay, appeared in all the immature control oocytes, but only 37% of mature oocytes showed DNA fragmentation. This DNA fragmentation was observed in 88.8% of the oocytes unfertilized after ICSI; furthermore, DNA fragmentation appeared as well in the sperm injected into the cytoplasm. Conclusion(s) The study has shown DNA fragmentation in human oocytes unfertilized after ICSI. The evidence is confirmed as well in control oocytes, free from in vitro culture or manipulation stress. Caspase-3 immunoassay suggests the presence of apoptosis. The high percentage of oocytes demonstrating DNA fragmentation in the unfertilized oocytes could be correlated with fertilization failure. |
| Databáze: | OpenAIRE |
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