Smoothened Inhibition Leads to Decreased Proliferation and Induces Apoptosis in Esophageal Adenocarcinoma Cells
| Autor: | Haris Zahoor, Usha Malhotra, Blair A. Jobe, Toshitaka Hoppo, Rory Makielski, Lori A. Kelly, Christina L. Rotoloni, Juliann E. Kosovec, Astha Bhatt, Yoshihiro Komatsu, Ali H. Zaidi |
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| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
Esophageal Neoplasms Blotting Western Fluorescent Antibody Technique Apoptosis Adenocarcinoma Biology medicine.disease_cause Receptors G-Protein-Coupled Flow cytometry Cell Line Tumor medicine Humans Hedgehog Proteins Cell Proliferation medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Cell growth General Medicine Flow Cytometry Smoothened Receptor Oncology Cancer cell Cancer research Signal transduction Smoothened Carcinogenesis Signal Transduction |
| Zdroj: | Cancer Investigation. 31:480-489 |
| ISSN: | 1532-4192 0735-7907 |
| DOI: | 10.3109/07357907.2013.820317 |
| Popis: | The Hedgehog (Hh) pathway is known to be active in Barrett's carcinogenesis. Therefore, we evaluated the efficacy and underlying mechanisms of inhibition of cancer cell growth by the smoothened (Smo) antagonist BMS-833923 in esophageal adenocarcinoma (EAC) cell lines. Cell proliferation and apoptosis were evaluated by flow cytometry, Western blotting, immunofluorescence, and quantitative reverse transcription polymerase chain reactions. Results showed that the Smo antagonist led to reduced Hh pathway activity, resulting in decreased cell proliferation and induction of apoptosis via the intrinsic pathway in the esophageal cancer cells. In conclusion, the Smo antagonist may have application as an EAC chemotherapeutic agent. |
| Databáze: | OpenAIRE |
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