Ginseng total saponin modulates the changes of α-actinin-4 in podocytes induced by diabetic conditions
| Autor: | Su-Bin Seong, Ja-Ae Nam, Ji-Young Choi, Hye-Young Park, Tae-Sun Ha |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
podocyte
macromolecular substances Plasma protein binding Biochemistry Genetics and Molecular Biology (miscellaneous) Podocyte Diabetic nephropathy Ginseng lcsh:Botany medicine Cytoskeleton Actin Proteinuria business.industry diabetic nephropathy α-actinin-4 Panax ginseng ginseng total saponin medicine.disease Actin cytoskeleton lcsh:QK1-989 Cell biology medicine.anatomical_structure Complementary and alternative medicine Immunology medicine.symptom business Research Article Biotechnology |
| Zdroj: | Journal of Ginseng Research Journal of Ginseng Research, Vol 38, Iss 4, Pp 233-238 (2014) |
| ISSN: | 1226-8453 |
| DOI: | 10.1016/j.jgr.2014.05.004 |
| Popis: | Background: The actin cytoskeleton in podocytes is essential for the maintenance of its normal structure and function. Its disruption is a feature of podocyte foot-process effacement and is associated with proteinuria. α-Actinin-4 in podocytes serves as a linker protein binding the actin filaments of the cytoskeleton. Methods: To investigate the effect of ginseng total saponin (GTS) on the pathological changes of podocyte α-actinin-4 induced by diabetic conditions, we cultured mouse podocytes under normal glucose (5mM) or high glucose (HG, 30mM) conditions, with or without the addition of advanced glycosylation end products (AGE), and treated with GTS. Results: In confocal imaging, α-actinin-4 colocalized with the ends of F-actin fibers in cytoplasm, but diabetic conditions disrupted F-actin fibers and concentrated α-actinin-4 molecules at the peripheral cytoplasm. GTS upregulated α-actinin protein in a time- and dose-dependent manner, and suppressed the receptor for AGE levels in western blotting. Diabetic conditions, including HG, AGE, and both together, decreased cellular α-actinin-4 protein levels at 24 h and 48 h. Such quantitative and qualitative changes of α-actinin-4 protein induced by diabetic conditions were mitigated by GTS. Conclusion: These findings imply that both HG and AGE have an influence on the distribution and amount of α-actinin-4 in podocytes that can be recovered by GTS. |
| Databáze: | OpenAIRE |
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