Sequence and transfection of BoLA-DRB3 cDNAs
| Autor: | Elizabeth Glass, Douglas C. Fraser, Bernadette M. Dutia, Susan Craigmile, George C. Russell, R. A. Oliver |
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| Rok vydání: | 2000 |
| Předmět: |
DNA
Complementary Sequence analysis Genes MHC Class II Molecular Sequence Data Major histocompatibility complex Transfection Evolution Molecular Antibody Specificity Sequence Analysis Protein Complementary DNA Genetics Animals Amino Acid Sequence Peptide sequence Gene HLA-DR Antigen Alleles MHC class II biology Sequence Homology Amino Acid Haplotype Histocompatibility Antigens Class II Antibodies Monoclonal General Medicine HLA-DR Antigens Molecular biology Haplotypes biology.protein Animal Science and Zoology Cattle HLA-DRB3 Chains |
| Zdroj: | Animal genetics. 31(3) |
| ISSN: | 0268-9146 |
| Popis: | Bovine MHC (BoLA-) DRB3 alleles encoded by the DH8A, DH22A and DH24A class II haplotypes were cloned from cDNA and characterized by sequence analysis. Comparison with other full-length DRB3 sequences suggested that DRB3 alleles may have evolved through multiple lineages. All three BoLA-DRB3 alleles were shown to express on the surface of transfected cells, and the transfectants were used to define or confirm the class II specificity of a panel of monoclonal antibodies. |
| Databáze: | OpenAIRE |
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