Non-invasive metabolomic profiling of culture media of ICSI- and IVF-derived early developmental cattle embryos via Raman spectroscopy
| Autor: | Ping-Hua Cao, Wen-Xia Han, Fan Zhang, Hua Wu, Ya-Kun Xu, Xiao-Xia Li, Xue-Li Yu, Jun-Yi Chen, Ying-Hua Li |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
animal structures medicine.medical_treatment Fertilization in Vitro Biology Spectrum Analysis Raman Intracytoplasmic sperm injection Embryo Culture Techniques Andrology 03 medical and health sciences 0302 clinical medicine Endocrinology Human fertilization Food Animals Pregnancy medicine Animals Sperm Injections Intracytoplasmic Blastocyst reproductive and urinary physiology 030219 obstetrics & reproductive medicine In vitro fertilisation urogenital system Embryogenesis Embryo General Medicine Embryonic stem cell In vitro Culture Media 030104 developmental biology medicine.anatomical_structure embryonic structures Cattle Female Animal Science and Zoology therapeutics |
| Zdroj: | Animal Reproduction Science. 196:99-110 |
| ISSN: | 0378-4320 |
| DOI: | 10.1016/j.anireprosci.2018.07.001 |
| Popis: | The aim of the present study was to compare differences in composition between in vitro cultured early developmental embryos resulting from either in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). Non-invasive metabolomic profiling of culture media was conducted with laser tweezer Raman spectroscopy (LTRS), providing molecular information that was used to aid the diagnosis or treatment of embryos that were adversely affected by ICSI treatment, ultimately improving the ICSI embryonic developmental potential. Cattle embryos were generated via ICSI and IVF with development to the 2-, 4-, 8-, 16-,32-cell, and blastocyst stages with individual in vitro culturing occurring for 4 h. The culture media for embryos in different developmental stages were separately analyzed using LTRS. The resulting composition of culture media used for culturing IVF- and ICSI-derived embryos was mainly altered in contents of carbohydrates, lipids, DNA, and proteins. Bands at 1004 cm−1 (phenylalanine) and 1529 cm−1 (-C = C-carotenoid) had specific patterns related to the metabolicactivity of embryos; using LTRS, and these may be considered as biomarkers for embryonic development. Furthermore, the vibrations of lipids at different stages increased more with assessment of ICSI culture media than in IVF media. Discriminant function analysis can be utilized for the classification of culture media used for culture of ICSI- and IVF-derived embryos. In conclusion, LTRS can be used for development of an independent assay to assess embryo status during both ICSI and IVF procedures, which provides novel insights into differences in structure and components of single cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect