Actin Waves Do Not Boost Neurite Outgrowth in the Early Stages of Neuron Maturation
| Autor: | Luisa M. R. Napolitano, Dan Cojoc, Vincent Torre, Simone Mortal, Elisa D’Este, Federico Iseppon, Andrea Perissinotto |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Neurite Motility Settore BIO/11 - Biologia Molecolare macromolecular substances RhoGTPases lcsh:RC321-571 03 medical and health sciences Cellular and Molecular Neuroscience Actin waves (AWs) B-cyclodextrin Growth cones (GCs) Myosin IIB Myosin medicine Axon Growth cone lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Actin Original Research Chemistry actin waves (AWs) Cell biology 030104 developmental biology medicine.anatomical_structure β-cyclodextrin Neuron maturation growth cones (GCs) Elongation Neuroscience |
| Zdroj: | Frontiers in Cellular Neuroscience Frontiers in cellular neuroscience 11 (2017). doi:10.3389/fncel.2017.00402 info:cnr-pdr/source/autori:Mortal S.; Iseppon F.; Perissinotto A.; D'Este E.; Cojoc D.; Napolitano L.M.R.; Torre V./titolo:Actin waves do not boost neurite outgrowth in the early stages of neuron maturation/doi:10.3389%2Ffncel.2017.00402/rivista:Frontiers in cellular neuroscience/anno:2017/pagina_da:/pagina_a:/intervallo_pagine:/volume:11 Frontiers in Cellular Neuroscience, Vol 11 (2017) |
| ISSN: | 1662-5102 |
| Popis: | During neurite development, Actin Waves (AWs) emerge at the neurite base and move up to its tip, causing a transient retraction of the Growth Cone (GC). Many studies have shown that AWs are linked to outbursts of neurite growth and, therefore, contribute to the fast elongation of the nascent axon. Using long term live cell-imaging, we show that AWs do not boost neurite outgrowth and that neurites without AWs can elongate for several hundred microns. Inhibition of Myosin II abolishes the transient GC retraction and strongly modifies the AWs morphology. Super-resolution nanoscopy shows that Myosin IIB shapes the growth cone-like AWs structure and is differently distributed in AWs and GCs. Interestingly, depletion of membrane cholesterol and inhibition of Rho GTPases decrease AWs frequency and velocity. Our results indicate that Myosin IIB, membrane tension, and small Rho GTPases are important players in the regulation of the AW dynamics. Finally, we suggest a role for AWs in maintaining the GCs active during environmental exploration. |
| Databáze: | OpenAIRE |
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