Effect of chlorhexidine/thymol and fluoride varnishes on dental biofilm formation in vitro
| Autor: | Yasuo Takeuchi, Bernhard Guggenheim, P. Baehni, Anna Filieri |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Saliva
Varnish Dental Plaque Statistics Nonparametric Microbiology chemistry.chemical_compound medicine Humans Fluorides Topical General Dentistry Thymol Microbial Viability biology Chemistry Chlorhexidine Biofilm biology.organism_classification Cariostatic Agents stomatognathic diseases Streptococcus oralis visual_art Anti-Infective Agents Local visual_art.visual_art_medium Actinomyces naeslundii Fluoride medicine.drug |
| Zdroj: | European Journal of Oral Sciences. 115:468-472 |
| ISSN: | 1600-0722 0909-8836 |
| DOI: | 10.1111/j.1600-0722.2007.00493.x |
| Popis: | This study evaluated the effect of chlorhexidine/thymol (CHX/T) and fluoride (F) varnishes on biofilm formation in vitro. Hydroxyapatite discs coated with varnish were first immersed in saline for 0, 3, 7 or 14 d, then immersed in pasteurized saliva. The discs were incubated for 20 h with a bacterial suspension containing Actinomyces naeslundii, Fusobacterium nucleatum, Streptococcus oralis, and Veillonella dispar. Uncoated discs were used as controls. Growth of bacteria on the discs was evaluated by culture and by scanning electron microscopy (SEM). Bacterial vitality was examined by fluorescence staining. In the CHX/T-treated group, bacterial accumulation was delayed, and the total number of bacteria was significantly lower than in the controls. In the F-treated group, the total number of bacteria did not differ from the control, although the number of S. oralis was lower. Bacterial vitality in the CHX/T and F groups did not differ from that in the controls. The total number of bacteria on the CHX/T-treated discs immersed in saline was significantly higher than that on the non-immersed discs. Biofilm development was inhibited by the CHX/T varnish but not by the F varnish. The effect of the CHX/T varnish decreased following the immersion of discs in saline. |
| Databáze: | OpenAIRE |
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