Immobilization and long-term culturing of mouse embryonic stem cells in collagen-chitosan gel matrix
| Autor: | E. F. Vikhlyantseva, B. K. Gavrilyuk, G. A. Davydova, I. I. Selezneva, I. V. Savintseva |
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| Rok vydání: | 2007 |
| Předmět: |
Cell Culture Techniques
Cell- and Tissue-Based Therapy macromolecular substances Embryoid body Matrix (biology) Polysaccharide General Biochemistry Genetics and Molecular Biology Chitosan chemistry.chemical_compound Mice medicine Animals Blastocyst Embryonic Stem Cells chemistry.chemical_classification technology industry and agriculture Hydrogels General Medicine Embryonic stem cell Molecular biology Cell biology Mice Inbred C57BL medicine.anatomical_structure chemistry Self-healing hydrogels Mice Inbred CBA Collagen Stem cell |
| Zdroj: | Bulletin of experimental biology and medicine. 142(1) |
| ISSN: | 0007-4888 |
| Popis: | We propose a method of creation of a 3D matrix consisting of native collagen fibers and natural polysaccharide chitosan. The collagen-chitosan hydrogels maintain viability and prolipherative activity of embryonic stem cells obtained from internal cells of mouse blastocyst. The proposed system forming hydrogels in situ can be used in cell therapy for immobilization and targeted delivery of stem cells. |
| Databáze: | OpenAIRE |
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