Biases in human papillomavirus genotype prevalence assessment associatedwith commonly used consensus primers
| Autor: | Jo L.K. Cheung, Mamie Hui, Tak Hong Cheung, Ann O. Y. Tam, Margaret Ip, May M.Y. Yu, Keith W.K. Lo, Ka Fai To, Paul K.S. Chan, So Fan Yim, Yick Fu Wong, Denise P. C. Chan |
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| Rok vydání: | 2005 |
| Předmět: |
Cancer Research
medicine.medical_specialty Genotype Uterine Cervical Neoplasms Cancer Vaccines Sensitivity and Specificity Virus law.invention law Epidemiology Prevalence medicine Humans Papillomaviridae Polymerase chain reaction DNA Primers Cervical cancer business.industry Papillomavirus Infections Reproducibility of Results Consensus primer medicine.disease Virology Oncology Carcinoma Squamous Cell Female Viral disease Primer (molecular biology) business |
| Zdroj: | International Journal of Cancer. 118:243-245 |
| ISSN: | 1097-0215 0020-7136 |
| DOI: | 10.1002/ijc.21299 |
| Popis: | Consensus primers targeting human papillomaviruses (HPVs) have biases in sensitivity toward certain HPV types. We applied 3 primer sets (GP5+/6+, MY09/11, PGMY09/11) in parallel on 120 Chinese cervical cancer specimens. GP5+/6+ exhibited a poor sensitivity for HPV52, for which the prevalence among squamous cell cervical cancer was underestimated from 14.6% to 0%. The fact that HPV52 should rank second in prevalence among squamous cell cervical carcinoma in Hong Kong could be missed if GP5+/6+, a worldwide commonly used primer set, was selected for HPV detection. Biases in HPV type-specific sensitivity may result in misprioritization of vaccine candidates. |
| Databáze: | OpenAIRE |
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