Effects of cerium oxide nanoparticles on differentiated/undifferentiated human intestinal Caco-2 cells
| Autor: | Ricard Marcos, Alba García-Rodríguez, Adriana Sampayo-Reyes, Constanza Cortés, Laura Vila, Alba Hernández, Noel Xamena, Antonia Velázquez |
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| Rok vydání: | 2017 |
| Předmět: |
Cell Membrane Permeability
Cell Survival Cellular differentiation media_common.quotation_subject Cell Metal Nanoparticles 02 engineering and technology 010501 environmental sciences Toxicology medicine.disease_cause 01 natural sciences law.invention Confocal microscopy law medicine Humans Intestinal Mucosa Particle Size Internalization 0105 earth and related environmental sciences media_common Microscopy Confocal Chemistry Cell Membrane Cell Differentiation General Medicine Cerium Apical membrane 021001 nanoscience & nanotechnology Comet assay Intestines medicine.anatomical_structure Caco-2 Biophysics Caco-2 Cells 0210 nano-technology Genotoxicity DNA Damage |
| Zdroj: | Chemico-biological interactions. 283 |
| ISSN: | 1872-7786 |
| Popis: | Since ingestion constitute one of the main routes of nanoparticles (NPs) exposure, intestinal cells seems to be a suitable choice to evaluate their potential harmful effects. Caco-2 cells, derived from a human colon adenocarcinoma, have the ability to differentiate forming consistent cell monolayer structures. For these reasons Caco-2 cells, both in their undifferentiated or differentiated state, are extendedly used. We have used well-structured monolayers of differentiated Caco-2 cells, as a model of intestinal barrier, to evaluate potential harmful effects associated to CeO2NPs exposure via ingestion. Different parameters such as cell toxicity, monolayer integrity and permeability, cell internalization, translocation through the monolayer, and induction of DNA damage were evaluated. No toxic effects of CeO2NPs were observed, independently of the differentiated state of the Caco-2 cells. In the same way, no effects on the monolayer integrity/permeability were observed. Although important cell uptake was demonstrated in undifferentiated cells (by using confocal microscopy), CeO2NPs remained mostly attached to the apical membrane in the differentiated cells. In spite of this apparent lack of uptake in differentiated cells, translocation of CeO2NPs to the basolateral chamber was observed by using confocal microscopy. Finally no genotoxic effects were observed when the comet assay was used, although decreases in the levels of oxidized bases were observed, supporting the antioxidant role of CeO2NPs. |
| Databáze: | OpenAIRE |
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