Isolation and characterization of cDNA clones encoding a 18.8 kDa polypeptide, the product of the gene psaL, associated with photosystem I reaction center from spinach
| Autor: | R. G. Herrmann, K. Flieger, Ralf Oelmüller |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Chloroplasts
Sequence analysis Protein subunit Molecular Sequence Data Photosynthetic Reaction Center Complex Proteins Biological Transport Active Plant Science Photosystem I Complementary DNA Genetics Amino Acid Sequence RNA Messenger Cloning Molecular Peptide sequence biology Base Sequence Photosystem I Protein Complex Sequence Homology Amino Acid Nucleic acid sequence food and beverages RNA General Medicine Sequence Analysis DNA Plants biology.organism_classification Molecular biology Biochemistry Spinach Agronomy and Crop Science Protein Processing Post-Translational |
| Zdroj: | Plant molecular biology. 22(4) |
| ISSN: | 0167-4412 |
| Popis: | Several cDNA clones encoding subunit XI of photosystem I reaction center (PSI-L) have been isolated from two lambda gt11 expression libraries based on polyadenylated RNA of spinach seedlings illuminated for 4 and 16 h, respectively. The precursor polypeptide made from these recombinant DNAs in vitro can be efficiently imported into isolated spinach chloroplasts. It is correctly processed to the size of the authentic polypeptide and integrates into the photosystem I assembly. The 834 nucleotide sequence of the longest cDNA insert encodes a precursor polypeptide of 24 kDa (216 residues) and a mature protein of probably 18.8 kDa (169 residues). Hydropathy analysis suggests that the polypeptide contains two transmembrane segments. The protein appears to originate in a single-copy gene in spinach and to be decoded from RNA species of ca. 900 bases. |
| Databáze: | OpenAIRE |
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