The characterization of trans-pecos copperhead (Agkistrodon contortrix pictigaster) venom and isolation of two new dimeric disintegrins

Autor: Ifunanya Ogbata, Néstor L. Uzcátegui, Elda E. Sánchez, Alexis Rodríguez-Acosta, Elyse Grilli, Montamas Suntravat, Amber Goins, Andrea Alfonso, Robert Walls, Belsy Guerrero, Sara Lucena
Rok vydání: 2015
Předmět:
0301 basic medicine
Agkistrodon
Disintegrins
Bioengineering
Venom
Applied Microbiology and Biotechnology
Article
03 medical and health sciences
Fibrinolytic Agents
Cations
Cell Line
Tumor
Crotalid Venoms
Disintegrin
Gelatinase
Animals
Humans
Amino Acid Sequence
Chromatography
High Pressure Liquid
Cell Proliferation
Skin
Pharmacology
Agkistrodon contortrix pictigaster
Chromatography
030102 biochemistry & molecular biology
General Immunology and Microbiology
biology
Chemistry
Copperhead
General Medicine
biology.organism_classification
Chromatography
Ion Exchange
3. Good health
030104 developmental biology
Biochemistry
Gelatinases
comic_books
biology.protein
Platelet aggregation inhibitor
Electrophoresis
Polyacrylamide Gel
Rabbits
Protein Multimerization
comic_books.character
Fibrinolytic agent
Platelet Aggregation Inhibitors
Biotechnology
Zdroj: Biologicals : journal of the International Association of Biological Standardization. 44(4)
ISSN: 1095-8320
Popis: The vast amounts of toxins within the venom of snakes, while known to cause medical emergencies, display various biological functions. Trans-pecos copperhead (Agkistrodon contortrix pictigaster) crude venom separated by cation-exchange chromatography showed several fractions with fibrinolytic, hemorrhagic, gelatinase and platelet activities. Venom fractions 1, 2, 4, 5, and 12-17 contained fibrinolytic activity. Venom fractions 1, 2, 5 and 12-14 had hemorrhagic activity. Fractions 1, 2, 12, 13 and 17 contained gelatinase activity. Reverse-Phase C18 High Performance Liquid Chromatography was also used to purify and isolated disintegrins from this venom. Anti-platelet aggregation activity of the C18 fractions collected and performed on whole human blood showed that they inhibited platelet aggregation in presence of several agonists. Results from both SDS-PAGE and N-terminal sequencing determined that pictistatin 1 obtained from the Trans-Pecos copperhead venom was a dimeric disintegrin, and pictistatin 2 was a heterodimeric disintegrin. The molecules with anti-platelet activity could be considered in the development of more effective drugs, for numerous blood-related diseases such as stroke, heart attacks, thrombosis, and other medical conditions. In this study, we are presenting the first report of the purification, isolation, and partial characterization of two new dimeric disintegrins isolated from the venom of trans-pecos copperhead.
Databáze: OpenAIRE
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