Neoboutonia melleri var velutina Prain: in vitro and in vivo hepatoprotective effects of the aqueous stem bark extract on acute hepatitis models

Autor: Céline Rivière, Théophile Dimo, Thierry Hennebelle, Pierre Desreumaux, Anne Marie Endougou Effa, Emilie Gantier, Vincent Roumy, Pierre Kamtchouing, Laurent Dubuquoy
Přispěvatelé: Lille Inflammation Research International Center - U 995 (LIRIC), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Laboratoire de Physiologie Animale [Yaoundé, Cameroon], Université de Yaoundé I, Département de Biologie des Organismes Animaux [Douala, Cameroon], Université de Douala-Université de Douala [Cameroon], Groupe de Recherche Interdiscipinaire Innovation et Optimisation Thérapeutique - EA 4481 (GRIIOT), Université de Lille, Droit et Santé, Service des Maladies de l'Appareil Digestif et de la Nutrition [CHRU Lille], Hôpital Claude Huriez [Lille], CHU Lille-CHU Lille-Fédération Hospitalière de France-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Service de la Coopération et d’Action Culturelle de l’Ambassade de France au Cameroun (N° bourses/194/2010) and Inserm., Inserm, Université de Lille, CHU Lille, INRA, ISA, Univ. Artois, Univ. Littoral Côte d’Opale, Lille Inflammation Research International Center - U 995 [LIRIC], Institut Charles Viollette (ICV) - EA 7394 [ICV], BMC, BMC, Fédération Hospitalière de France-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Hôpital Claude Huriez [Lille], CHU Lille-CHU Lille
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
Antioxidant
medicine.medical_treatment
[SDV]Life Sciences [q-bio]
Protective Agents
Neoboutonia
Mice
03 medical and health sciences
chemistry.chemical_compound
In vivo
Mesh:Cytokines/metabolism
Mesh:Plant Bark/chemistry
Mesh:Euphorbiaceae/chemistry
Mesh:Animals
Mesh:Plant Extracts/chemistry
Mesh:Male
Mesh:Plant Extracts/pharmacology
Mesh:Carbon Tetrachloride/toxicity
Mesh:Protective Agents/chemistry
Mesh:Chemical and Drug Induced Liver Injury/metabolism
Mesh:Liver/drug effects
Mesh:Mice
Mesh:Hep G2 Cells
Mesh:Protective Agents/pharmacology
Mesh:Cytokines/analysis
Mesh:Inbred C57BL
Mesh:Humans
medicine
Animals
Humans
Acute hepatitis
Medicinal plants
Carbon Tetrachloride
Hepatitis
Liver injury
biology
Traditional medicine
Plant Extracts
Euphorbiaceae
Hep G2 Cells
lcsh:Other systems of medicine
General Medicine
lcsh:RZ201-999
biology.organism_classification
medicine.disease
3. Good health
Mice
Inbred C57BL
[SDV] Life Sciences [q-bio]
030104 developmental biology
Liver
Complementary and alternative medicine
chemistry
Phytochemical
Neoboutonia melleri var. velutina Prain
Plant Bark
Carbon tetrachloride
Cytokines
Chemical and Drug Induced Liver Injury
Research Article
Preventive treatment
Zdroj: BMC Complementary and Alternative Medicine
BMC Complementary and Alternative Medicine, 2017, 18 (1), pp.24. ⟨10.1186/s12906-018-2091-2⟩
BMC Complementary and Alternative Medicine, Vol 18, Iss 1, Pp 1-18 (2018)
BMC Complementary and Alternative Medicine, BioMed Central, 2017, 18 (1), pp.24. ⟨10.1186/s12906-018-2091-2⟩
ISSN: 1472-6882
Popis: Background Hepatitis is a liver inflammation caused by different agents and remains a public health problem worldwide. Medicinal plants are an important source of new molecules being considered for treatment of this disease. Our work aims at evaluating the hepatoprotective properties of Neoboutonia velutina, a Cameroonian medicinal plant. Methods The aqueous extract has been prepared using phytochemical methods. HepG2 cells were used to assess anti-inflammatory properties of the extract at different concentrations. Acute hepatitis models (Carbon tetrachloride and Concanavalin A) were performed in mice receiving or not receiving, different extract doses by gavage. Liver injury was assessed using histology, transaminases and pro-inflammatory markers. Extract antioxidant and radical scavenging capacities were evaluated. Results The extract led to a significant decrease in pro-inflammatory cytokine expression in vitro and to a remarkable protection of mice from carbon tetrachloride-induced liver injury, as shown by a significant decrease in dose-dependent transaminases level. Upon extract treatment, inflammatory markers were significantly decreased and liver injuries were limited as well. In the Concanavalin A model, the extract displayed weak effects. Conclusions Taking into account underlying mechanisms in both hepatitis models, we demonstrate the extract’s radical scavenging capacity. Neoboutonia velutina displays a potent hepatoprotective effect mediated through radical scavenging properties. Electronic supplementary material The online version of this article (10.1186/s12906-018-2091-2) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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