Analysis of the flanking regions of the human alpha-lactalbumin gene responsible for position-effect independent expression
| Autor: | Kenji Sekikawa, Ri-ichi Takahashi, Tatsuo Muramatsu, Harumichi Yamanaka, Yoshihiro Fujiwara, Masumi Hirabayashi, Masatsugu Ueda |
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| Rok vydání: | 2003 |
| Předmět: |
Chromosomes
Artificial Bacterial 5' Flanking Region 5' flanking region Molecular Sequence Data Gene Dosage Biology Gene dosage Polymerase Chain Reaction Animals Genetically Modified Genetics Animals Deoxyribonuclease I Humans Gene Chromosomes Artificial Yeast Locus control region Regulation of gene expression Bacterial artificial chromosome Binding Sites Nucleic acid sequence General Medicine DNA Sequence Analysis DNA Molecular biology Rats Position effect Gene Expression Regulation Lactalbumin |
| Zdroj: | Gene. 305(1) |
| ISSN: | 0378-1119 |
| Popis: | Transgenic rats with the 130 kb bacterial artificial chromosome construct bLA, including the alpha-lactalbumin gene, had position-independent and copy number-dependent expression, which confirmed previous experiments using the 210 kb yeast artificial construct, yLALBA. To identify elements that confer a position effect, we compared the yLALBA and bLA sequences. yLALBA was chimeric. A common 32 kb region was identified and the total nucleotide sequence was determined. We previously analyzed transgenic rats using polymerase chain reaction to compare the integrity and expression of the transgenes. The -6 to +9 kb region is considered to be necessary for position-independent expression. Transgenic rats lacking the -3.4 to -0.85 kb region had a severe position effect. This 2.5 kb region contains two DNaseI hypersensitive sites at -1.0 and -2.8 kb. The 2.5 kb region is proposed to be a locus control region of the human alpha-lactalbumin gene. |
| Databáze: | OpenAIRE |
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