Flavonoid–DNA binding studies and thermodynamic parameters
| Autor: | Azra Yaqub, Claus Jacob, Lalla A. Ba, Naveed Kausar Janjua, Misbah Mumtaz, Amber Shaheen, Fouzia Perveen, Sana Sabahat, Hamdoon A. Mohammed |
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| Rok vydání: | 2011 |
| Předmět: |
Flavonoids
chemistry.chemical_classification Molecular model medicine.diagnostic_test Chemistry Stereochemistry Relative viscosity Flavonoid food and beverages Viscometer DNA Binding constant Atomic and Molecular Physics and Optics Analytical Chemistry chemistry.chemical_compound Flavonoid derivatives Spectrophotometry medicine Animals Thermodynamics Spectrophotometry Ultraviolet Chickens Instrumentation Spectroscopy |
| Zdroj: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 79:1600-1604 |
| ISSN: | 1386-1425 |
| DOI: | 10.1016/j.saa.2011.05.018 |
| Popis: | Interactional studies of new flavonoid derivatives (Fl) with chicken blood ds.DNA were investigated spectrophotometrically in DMSO-H2O (9:1 v/v) at various temperatures. Spectral parameters suggest considerable binding between the flavonoid derivatives studied and ds.DNA. The binding constant values lie in the enhanced-binding range. Thermodynamic parameters obtained from UV studies also point to strong spontaneous binding of Fl with ds.DNA. Viscometric studies complimented the UV results where a small linear increase in relative viscosity of the DNA solution was observed with added optimal flavonoid concentration. An overall mixed mode of interaction (intercalative plus groove binding) is proposed between DNA and flavonoids. Conclusively, investigated flavonoid derivatives are found to be strong DNA binders and seem to be promising drug candidates like their natural analogues. |
| Databáze: | OpenAIRE |
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