Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy
| Autor: | Taylor Hinsdale, Maryellen L. Giger, Kristen C. Maitland, Bilal H. Malik, John M. Wright, Shuna Cheng, Javier A. Jo, Oscar R. Benavides, Paras B. Patel |
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| Rok vydání: | 2020 |
| Předmět: |
Mild Dysplasia
Fluorescence-lifetime imaging microscopy Pathology medicine.medical_specialty Science 01 natural sciences Article Fluorescence imaging 010309 optics 03 medical and health sciences 0302 clinical medicine In vivo Cheek pouch 0103 physical sciences medicine Animals Oral mucosa Oral Dysplasia Microscopy Multidisciplinary Mesocricetus Chemistry Squamous Cell Carcinoma of Head and Neck Oral cancer medicine.disease Fluorescence medicine.anatomical_structure Microscopy Fluorescence Dysplasia 030220 oncology & carcinogenesis Medicine Mouth Neoplasms Applied optics NADP |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
| ISSN: | 2045-2322 |
| Popis: | We demonstrate that structured illumination microscopy has the potential to enhance fluorescence lifetime imaging microscopy (FLIM) as an early detection method for oral squamous cell carcinoma. FLIM can be used to monitor or detect changes in the fluorescence lifetime of metabolic cofactors (e.g. NADH and FAD) associated with the onset of carcinogenesis. However, out of focus fluorescence often interferes with this lifetime measurement. Structured illumination fluorescence lifetime imaging (SI-FLIM) addresses this by providing depth-resolved lifetime measurements, and applied to oral mucosa, can localize the collected signal to the epithelium. In this study, the hamster model of oral carcinogenesis was used to evaluate SI-FLIM in premalignant and malignant oral mucosa. Cheek pouches were imaged in vivo and correlated to histopathological diagnoses. The potential of NADH fluorescence signal and lifetime, as measured by widefield FLIM and SI-FLIM, to differentiate dysplasia (pre-malignancy) from normal tissue was evaluated. ROC analysis was carried out with the task of discriminating between normal tissue and mild dysplasia, when changes in fluorescence characteristics are localized to the epithelium only. The results demonstrate that SI-FLIM (AUC = 0.83) is a significantly better (p-value = 0.031) marker for mild dysplasia when compared to widefield FLIM (AUC = 0.63). |
| Databáze: | OpenAIRE |
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