Direct Analysis of Gene Synthesis Reactions Using Solid-State Nanopores
| Autor: | Spencer Carson, Peter A. Carr, Scott T. Wick, Carlos A. Aguilar, Meni Wanunu |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
General Engineering
Cellular functions Solid-state General Physics and Astronomy Nanotechnology DNA Sequence Analysis DNA Biology Chemical synthesis Article Nanopore Synthetic biology Kinetics Nanopores Genes HIV Protease DNA nanotechnology General Materials Science Synthetic Biology Biochemical engineering Direct analysis Gene synthesis |
| Zdroj: | ACS nano. 9(12) |
| ISSN: | 1936-086X |
| Popis: | Synthetic nucleic acids offer rich potential to understand and engineer new cellular functions, yet an unresolved limitation in their production and usage is deleterious products, which restrict design complexity and add cost. Herein, we employ a solid-state nanopore to differentiate molecules of a gene synthesis reaction into categories of correct and incorrect assemblies. This new method offers a solution that provides information on gene synthesis reactions in near-real time with higher complexity and lower costs. This advance can permit insights into gene synthesis reactions such as kinetics monitoring, real-time tuning, and optimization of factors that drive reaction-to-reaction variations as well as open venues between nanopore-sensing, synthetic biology, and DNA nanotechnology. |
| Databáze: | OpenAIRE |
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