Effect of trehalose on cryopreservation of pure peripheral blood stem cells
| Autor: | Cristina Colarossi, Gabriella Denti, Simona Buccheri, Daniela Martinetti, Luisa Vicari, Lorenzo Memeo |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030102 biochemistry & molecular biology Cryoprotectant General Neuroscience Cell Articles General Medicine Biology medicine.disease Trehalose General Biochemistry Genetics and Molecular Biology Cryopreservation Cell biology 03 medical and health sciences chemistry.chemical_compound Haematopoiesis 030104 developmental biology medicine.anatomical_structure chemistry medicine Viability assay General Pharmacology Toxicology and Pharmaceutics Stem cell Cell damage |
| Zdroj: | Biomedical Reports. 6:314-318 |
| ISSN: | 2049-9442 2049-9434 |
| DOI: | 10.3892/br.2017.859 |
| Popis: | Stem cells are an important tool for the study of hematopoiesis. Despite developments in cryopreservation, post-thaw cell death remains a considerable problem. Cryopreservation protocol should limit cell damage due to freezing and ensure the recovery of the functional cell characteristics after thawing. Thus, the use of cryoprotectants is essential. In particular, the efficacy of trehalose has been reported for clinical purposes in blood stem cells. The aim of the current study was to establish an efficient method for biological research based on the use of trehalose, to cryopreserve pure peripheral blood stem cells. The efficacy of trehalose was assessed in vitro and the cell viability was evaluated. The data indicate that trehalose improves cell survival after thawing compared with the standard freezing procedure. These findings could suggest the potential for future trehalose application for research purposes in cell cryopreservation. |
| Databáze: | OpenAIRE |
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