The impact of Wnt signalling and hypoxia on osteogenic and cementogenic differentiation in human periodontal ligament cells
| Autor: | Yinghong Zhou, Yin Xiao, Thor Friis, Jiang-Wu Yao, Shuigen Li, Bin Shi, Jin Shao |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
0301 basic medicine Cancer Research Adolescent Periodontal Ligament Cellular differentiation Biology Biochemistry osteogenesis Wnt Young Adult 03 medical and health sciences 0302 clinical medicine Genetics medicine Humans Periodontal fiber Cementum Wnt Signaling Pathway Molecular Biology Transcription factor Cells Cultured Cell Proliferation Oncogene hypoxia Wnt signaling pathway Cell Differentiation Articles Hypoxia-Inducible Factor 1 alpha Subunit Cementogenesis Cell biology cementogenesis RUNX2 030104 developmental biology medicine.anatomical_structure Oncology Culture Media Conditioned 030220 oncology & carcinogenesis Molecular Medicine |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | Cementum is a periodontal support tissue that is directly connected to the periodontal ligament. It shares common traits with bone tissues, however, unlike bone, the cementum has a limited capacity for regeneration. As a result, following damage the cementum rarely, if ever, regenerates. Periodontal ligament cells (PDLCs) are able to differentiate into osteoblastic and cementogenic lineages according to specific local environmental conditions, including hypoxia, which is induced by inflammation or activation of the Wnt signalling pathway by local loading. The interactions between the Wnt signalling pathway and hypoxia during cementogenesis are of particular interest to improve the understanding of periodontal tissue regeneration. In the present study, osteogenic and cementogenic differentiation of PDLCs was investigated under hypoxic conditions in the presence and absence of Wnt pathway activation. Protein and gene expression of the osteogenic markers type 1 collagen (COL1) and runt‑related transcription factor 2 (RUNX2), and cementum protein 1 (CEMP1) were used as markers for osteogenic and cementogenic differentiation, respectively. Wnt signalling activation inhibited cementogenesis, whereas hypoxia alone did not affect PDLC differentiation. However, hypoxia reversed the inhibition of cementogenesis that resulted from overexpression of Wnt signalling. Cross-talk between hypoxia and Wnt signalling pathways was, therefore, demonstrated to be involved in the differentiation of PDLCs to the osteogenic and cementogenic lineages. In summary, the present study suggests that the differentiation of PDLCs into osteogenic and cementogenic lineages is partially regulated by the Wnt signalling pathway and that hypoxia is also involved in this process. |
| Databáze: | OpenAIRE |
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